TY - JOUR
T1 - Role of the siderophore transporter SirABC in the Staphylococcus aureus resistance to oxidative stress
AU - Nobre, Lígia S.
AU - Saraiva, Lígia M.
N1 - WOS:000338288100008
PY - 2014
Y1 - 2014
N2 - In Staphylococcus aureus, the intracellular siderophore staphyloferrin B, which has been shown to chelate iron-bound to serum transferrin, is transported into cells by the SirABC system. In this work, we have analysed the role of the Sir transporter under stress conditions that resemble those imposed by the mammalian innate immune system. We show that exposure of S. aureus to oxidative and nitrosative stress generated by hydrogen peroxide and S-nitrosoglutathione, respectively, induced the expression of the sirA gene. The disruption of the sir operon led to a strain with lower viability and decreased resistance to oxidative stress. S. aureus sir null mutant was also analysed during infection of murine macrophages and shown to contribute to S. aureus survival inside macrophages. Altogether, our results indicate that the Sir transport system confers protection against reactive oxygen species, therefore, contributing to the virulence of S. aureus.
AB - In Staphylococcus aureus, the intracellular siderophore staphyloferrin B, which has been shown to chelate iron-bound to serum transferrin, is transported into cells by the SirABC system. In this work, we have analysed the role of the Sir transporter under stress conditions that resemble those imposed by the mammalian innate immune system. We show that exposure of S. aureus to oxidative and nitrosative stress generated by hydrogen peroxide and S-nitrosoglutathione, respectively, induced the expression of the sirA gene. The disruption of the sir operon led to a strain with lower viability and decreased resistance to oxidative stress. S. aureus sir null mutant was also analysed during infection of murine macrophages and shown to contribute to S. aureus survival inside macrophages. Altogether, our results indicate that the Sir transport system confers protection against reactive oxygen species, therefore, contributing to the virulence of S. aureus.
UR - http://www.scopus.com/inward/record.url?scp=84904014972&partnerID=8YFLogxK
U2 - 10.1007/s00284-014-0567-y
DO - 10.1007/s00284-014-0567-y
M3 - Article
C2 - 24682218
AN - SCOPUS:84904014972
VL - 69
SP - 164
EP - 168
JO - Current Microbiology
JF - Current Microbiology
SN - 0343-8651
IS - 2
ER -