Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli

Ramakanth Neeli-Venkata, Antti Martikainen, Abhishekh Gupta, Nadia Gonçalves, Jose Fonseca, André S. Ribeiro

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12 Citations (Scopus)
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Abstract

UNLABELLED: Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis also changes between conditions and in agreement with the nucleoid exclusion phenomenon. Consequently, the fraction of aggregates at the midcell region prior to cell division differs between conditions, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, from the location of the peak of anisotropy in the aggregate displacement distribution, the nucleoid relative size, and the spatiotemporal aggregate distribution, we find that the exclusion of detectable aggregates from midcell is most pronounced in cells with mid-sized nucleoids, which are most common under optimal conditions. We conclude that the aggregate management mechanisms of E. coli are significantly robust but are not immune to stresses due to the tangible effect that these have on nucleoid size.

IMPORTANCE: Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. From live single-cell microscopy studies of the robustness of this process to various stresses known to affect nucleoid size, we find that nucleoid size and aggregate preferential locations change concordantly between conditions. Also, the degree of influence of the nucleoid on aggregate positioning differs between conditions, causing aggregate numbers at midcell to differ in cell division events, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, we find that aggregate segregation to the cell poles is most pronounced in cells with mid-sized nucleoids. We conclude that the energy-free process of the midcell exclusion of aggregates partially loses effectiveness under stressful conditions.

Original languageEnglish
Pages (from-to)898-906
Number of pages9
JournalJournal of Bacteriology
Volume198
Issue number6
DOIs
Publication statusPublished - Mar 2016

Keywords

  • Cell Division
  • Escherichia coli
  • Escherichia coli Proteins
  • Genes, Reporter
  • Luminescent Proteins
  • Microscopy, Fluorescence
  • Protein Aggregates
  • Staining and Labeling
  • Time-Lapse Imaging
  • Journal Article
  • Research Support, Non-U.S. Gov't

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  • Cite this

    Neeli-Venkata, R., Martikainen, A., Gupta, A., Gonçalves, N., Fonseca, J., & Ribeiro, A. S. (2016). Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli. Journal of Bacteriology, 198(6), 898-906. https://doi.org/10.1128/JB.00848-15