Rheology of living cells

R. Portela, J. M. Franco, P. L. Almeida, P. Patrício, R. G. Sobral, C. R. Leal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The mechanical behavior of living cells, during planktonic growth, has been thoroughly explored combining common biological techniques with rheology and rheo-imaging measurements. Under a shear flow, bacterial cultures of Staphylococcus aureus revealed a complex and rich rheological behavior not usually accessed in biological studies. In particular, in stationary shear flow, the viscosity increased during the exponential phase and returned close to its initial value at the late phase of growth, accompanied by the stabilization of the bacterial population. In oscillatory flow, the elastic and viscous moduli exhibited power-law behaviors whose exponents are dependent on the bacteria growth stage, and can be associated to a Soft Glassy Material behavior. These behaviors were framed in a microscopic model that suggests the formation of a dynamic web-like structure, where specific aggregation phenomena may occur, depending on growth stage and cell density. Furthermore, systematic measurements combining optical density and dry weight techniques presented new evidences, which confirmed that the observed cell aggregation patterns developed during growth, under shear, can not only be cell density dependent.

Original languageEnglish
Title of host publication6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538685068
DOIs
Publication statusPublished - 15 Apr 2019
Event6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Lisbon, Portugal
Duration: 22 Feb 201923 Feb 2019

Conference

Conference6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019
CountryPortugal
CityLisbon
Period22/02/1923/02/19

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  • Cite this

    Portela, R., Franco, J. M., Almeida, P. L., Patrício, P., Sobral, R. G., & Leal, C. R. (2019). Rheology of living cells. In 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings [8692466] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ENBENG.2019.8692466