TY - JOUR
T1 - RGD functionalized poly(ε-caprolactone)/poly(m-anthranilic acid) electrospun nanofibers as high-performing scaffolds for bone tissue engineering RGD functionalized PCL/P3ANA nanofibers
AU - Guler, Zeliha
AU - Silva, J. Carvalho
AU - Sezai Sarac, A.
N1 - Sem pdf conforme despacho.
Scientific and Technological Research Council of Turkey (TUBITAK) - 213M469; 2211C ;
Portuguese Foundation for Science and Technology (FCT)- PEst-OE/FIS/UI0068/2011
PY - 2017/2/11
Y1 - 2017/2/11
N2 - RGD functionalized nanofibers of poly(ε-caprolactone)/poly(m-anthranilic acid) (PCL/P3ANA) were fabricated to address the mechanical, structural and functional requirements of bone tissue engineering. Nanofibers containing the highest amount of P3ANA with more carboxyl groups for functionalization have exhibited higher surface area and better mechanical properties. FTIR-ATR and UV-visible measurements evidenced the covalent RGD immobilization. After RGD peptide immobilization, the surface properties of nanofibers changed as evidenced by contact angle and electrochemical impedance spectroscopy (EIS). The effects of RGD functionalized nanofibers (PCL/P3ANA-RGD) on Saos-2 cell attachment, proliferation, and osteogenic activity were investigated. PCL/P3ANA-RGD nanofibers favored cell attachment, proliferation, and osteogenic activity.
AB - RGD functionalized nanofibers of poly(ε-caprolactone)/poly(m-anthranilic acid) (PCL/P3ANA) were fabricated to address the mechanical, structural and functional requirements of bone tissue engineering. Nanofibers containing the highest amount of P3ANA with more carboxyl groups for functionalization have exhibited higher surface area and better mechanical properties. FTIR-ATR and UV-visible measurements evidenced the covalent RGD immobilization. After RGD peptide immobilization, the surface properties of nanofibers changed as evidenced by contact angle and electrochemical impedance spectroscopy (EIS). The effects of RGD functionalized nanofibers (PCL/P3ANA-RGD) on Saos-2 cell attachment, proliferation, and osteogenic activity were investigated. PCL/P3ANA-RGD nanofibers favored cell attachment, proliferation, and osteogenic activity.
KW - covalent immobilization
KW - Electrospun nanofiber
KW - in vitro cell studies
KW - poly(m-antranilic acid)
KW - RGD peptide
UR - http://www.scopus.com/inward/record.url?scp=84995566982&partnerID=8YFLogxK
U2 - 10.1080/00914037.2016.1190929
DO - 10.1080/00914037.2016.1190929
M3 - Article
AN - SCOPUS:84995566982
SN - 0091-4037
VL - 66
SP - 139
EP - 148
JO - International Journal of Polymeric Materials and Polymeric Biomaterials
JF - International Journal of Polymeric Materials and Polymeric Biomaterials
IS - 3
ER -