Reversible and Selective Interconversion of Hydrogen and Carbon Dioxide into Formate by a Semiartificial Formate Hydrogenlyase Mimic

Katarzyna P. Sokol, William E. Robinson, Ana R. Oliveira, Sonia Zacarias, Chong Yong Lee, Christopher Madden, Arnau Bassegoda, Judy Hirst, Inês A.C. Pereira, Erwin Reisner

Research output: Contribution to journalArticle

2 Citations (Scopus)
5 Downloads (Pure)

Abstract

The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H2ase) and produces H2 and CO2 from formate via mixed-acid fermentation in Escherichia coli. Here, we describe an electrochemical and a colloidal semiartificial FHL system that consists of an FDH and a H2ase immobilized on conductive indium tin oxide (ITO) as an electron relay. These in vitro systems benefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversion of formate to H2 and CO2 under ambient temperature and pressure. The hybrid systems provide a template for the design of synthetic catalysts and surpass the FHL complex in vivo by storing and releasing H2 on demand by interconverting CO2/H2 and formate with minimal bias in either direction.

Original languageEnglish
Pages (from-to)17498-17502
Number of pages5
JournalJournal of the American Chemical Society
Volume141
Issue number44
DOIs
Publication statusPublished - 4 Sep 2019

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