Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough

Antonio J. Neca, Rui Soares, Marta S. P. Carepo, Sofia R. Pauleta

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Abstract

We report the 94 % assignment of DVU2108, a protein belonging to the Orange Protein family, that in Desulfovibrio vulgaris Hildenborough forms a protein complex named the Orange Protein complex. This complex has been shown to be implicated in the cell division of this organism. DVU2108 is a conserved protein in anaerobic microorganisms and in Desulfovibrio gigas the homologous protein was isolated with a novel Mo-Cu cluster non-covalently attached to the polypeptide chain. However, the heterologously produced DVU2108 did not contain any bound metal. These assignments provide the means to characterize the interaction of DVU2108 with the proteins that form the Orange Protein complex using NMR methods.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalBiomolecular Nmr Assignments
Volume10
Issue number1
DOIs
Publication statusPublished - Apr 2016

Keywords

  • NMR assignment
  • Cell division
  • Novel Mo-Cu cluster
  • Desulfovibrio
  • Anaerobic bacteria
  • NMR CHEMICAL-SHIFTS
  • APO-FORM
  • CLUSTER
  • GIGAS
  • MO
  • CU

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