Residue-specific NH exchange rates studied by NMR diffusion experiments

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Abstract

We present a novel approach to the investigation of rapid (>2 s(-1)) NH exchange rates in proteins, based oil residue-specific diffusion measurements. H-1, N-15-DOSY-HSQC spectra are recorded in order to observe resolved amide proton signals for most residues of the protein. Human ubiquitin was used to demonstrate the proposed method. Exchange rates are derived directly from the decay data of the diffusion experiment by applying a model deduced from the assumption of a two-site exchange with water and the "pure" diffusion coefficients of water and protein. The "pure" diffusion coefficient of the protein is determined in an experiment with selective excitation of the amide protons in order to suppress the influence of magnetization transfer from water to amide protons on the decay data. For rapidly exchanging residues a comparison of our results With the exchange rates obtained in a MEXICO experiment showed good agreement. Molecular dynamics (MD) and quantum mechanical calculations were performed to find molecular parameters correlating with the exchangeability of the NH protons. The RMS fluctuations of the amide protons, obtained from the MD simulations, together with the NH coupling constants provide a bilinear model which shows a good correlation with the experimental NH exchange rates. (C) 2007 Elsevier Inc. All rights reserved.
Original languageUnknown
Pages (from-to)97-104
JournalJournal Of Magnetic Resonance
Volume187
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007

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