Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs

S. J. Semião-Santos, P Abranches, M. C D Silva-Pereira, G. M. Santos-Gomes, J. P. Fernandes, J. C M Vetter

Research output: Contribution to journalArticle

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Abstract

A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude.

Original languageEnglish
Pages (from-to)747-750
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
Volume91
Issue number6
Publication statusPublished - Nov 1996

Fingerprint

Agglutination Tests
Leishmaniasis
Dogs
Canidae
Leishmania infantum
Portugal
Seroepidemiologic Studies
Serologic Tests
Infection
Fluorescent Antibody Technique
Reading
Population

Keywords

  • Canine
  • Direct agglutination test
  • Immunofluorescence technique
  • Leishmaniasis
  • Vulpine

Cite this

Semião-Santos, S. J., Abranches, P., Silva-Pereira, M. C. D., Santos-Gomes, G. M., Fernandes, J. P., & Vetter, J. C. M. (1996). Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs. Memorias do Instituto Oswaldo Cruz, 91(6), 747-750.
Semião-Santos, S. J. ; Abranches, P ; Silva-Pereira, M. C D ; Santos-Gomes, G. M. ; Fernandes, J. P. ; Vetter, J. C M. / Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs. In: Memorias do Instituto Oswaldo Cruz. 1996 ; Vol. 91, No. 6. pp. 747-750.
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Semião-Santos, SJ, Abranches, P, Silva-Pereira, MCD, Santos-Gomes, GM, Fernandes, JP & Vetter, JCM 1996, 'Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs', Memorias do Instituto Oswaldo Cruz, vol. 91, no. 6, pp. 747-750.

Reliability of serological methods for detection of leishmaniasis in Portuguese domestic and wild reservoirs. / Semião-Santos, S. J.; Abranches, P; Silva-Pereira, M. C D; Santos-Gomes, G. M.; Fernandes, J. P.; Vetter, J. C M.

In: Memorias do Instituto Oswaldo Cruz, Vol. 91, No. 6, 11.1996, p. 747-750.

Research output: Contribution to journalArticle

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AU - Semião-Santos, S. J.

AU - Abranches, P

AU - Silva-Pereira, M. C D

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AB - A direct agglutination test (DAT) and an immunofluorescence technique (IFAT) were compared for detection of Leishmania infantum infection in 43 dogs and five foxes from Alto-Douro and Arrábida, two known endemic areas in Portugal. In four dogs with proved canine leishmaniasis, both DAT and IFAT showed positive readings (titres ≥1:320 and ≥1:128). Of 34 samples collected from apparently healthy dogs, ten were positive by both serological tests and eight were serologically positive by one test or the other. Three foxes out of five captured in this area, scored titres indicative of leishmaniasis in both DAT and IFAT. The concordance between DAT and IFAT in all collected samples (48) was 81.25%. Considering these and previous studies in the adjacent Mediterranean areas, the seroprevalence ofL. infantum infection in the canine and vulpine populations appear to be of high magnitude.

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