Regulation of expression of the amino acid transporter gene BAP3 in Saccharomyces cerevisiae

Marco De Boer, Jan Paul Bebelman, Paula M. Gonçalves, Jan Maat, Harm Van Heerikhuizen, Rudi J. Planta

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Abstract

The BAP3 gene of Saccharomyces cerevisiae encodes a protein with a high similarity to the BAP2 gene product, a high-affinity permease for branched-chain amino acids. In this paper, we show that, like BAP2, the expression of the BAP3 gene in S. cerevisiae is induced by the addition of branched-chain amino acids to the medium. Unexpectedly, most other naturally occurring L-amino acids found in proteins (with the exception of proline, lysine, arginine and histidine) have the same effect on the expression of BAP3. The induction of BAP3 expression appears to be dependent on Stp1p, a nuclear protein, previously shown to be involved in pre-tRNA maturation and also required for the expression of BAP2, as induction is no longer observed in an stp1- mutant. The transcriptional regulator Leu3p is not involved in the induction of BAP3 expression, but may act as a repressor of BAP3 expression in the absence of leucine, as can be inferred from a transcriptional analysis in a Δleu3 mutant. By extensive deletion analysis of the BAP3 promoter fused to a GUS reporter, as well as by fusions of different parts of the BAP3 promoter to a LacZ reporter, we have found that a portion of the BAPS promoter from -418 to -392 relative to the ATG start codon is both necessary and sufficient for the Stp1p-dependent induction of BAP3 expression by (most) amino acids. We have therefore named this sequence UAS(aa) (amino acid-dependent upstream activator sequence). Neither Stp1p nor Leu3p appear to bind to the UAS(aa), at least in vitro, as judged from gel retardation assays. Sequences similar to the UAS(aa) can be found in the promoters of BAP2, PTR2 and TAT1; genes that, like BAP3, encode permeases inducible by amino acids, suggesting that amino acid induction of all these genes is exerted via a common mechanism.

Original languageEnglish
Pages (from-to)603-613
Number of pages11
JournalMolecular Microbiology
Volume30
Issue number3
DOIs
Publication statusPublished - 1998

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Keywords

  • transfer rna
  • amino acid
  • arginine
  • carrier protein
  • fungal protein
  • gene product
  • leucine
  • lysine
  • permease
  • proline

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