Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774

Clara Reis; Ricardo O. Louro; Isabel Pacheco; Teresa Catarino; David L. Turner; António V. Xavier

doi:10.1016/S0020-1693(02)00951-9

Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774

Clara Reis, Ricardo O. Louro, Isabel Pacheco, Teresa Catarino, David L. Turner, António V. Xavier

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pK_a^ox and pK_a^red values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.

Original language	English
Pages (from-to)	248-252
Number of pages	5
Journal	Inorganica Chimica Acta
Volume	339
DOIs	https://doi.org/10.1016/S0020-1693(02)00951-9
Publication status	Published - 15 Nov 2002

Keywords

Desulfovibrio desulfuricans
Electrostatic models
Energy transduction
Nine-haem cytochome c
Redox titrations
Redox-Bohr effect

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title = "Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774",

abstract = "Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pKaox and pKared values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.",

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T1 - Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774

AU - Reis, Clara

AU - Louro, Ricardo O.

AU - Pacheco, Isabel

AU - Catarino, Teresa

AU - Turner, David L.

AU - Xavier, António V.

PY - 2002/11/15

Y1 - 2002/11/15

N2 - Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pKaox and pKared values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.

AB - Redox titrations followed by visible spectroscopy were performed at various pH values with the nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774 (Dd27774). Macroscopic midpoint reduction potentials of the haems and pKaox and pKared values of acid-base centres that interact with the redox centres were estimated by using a thermodynamic model, in which the haems are grouped in three sets, and two acid-base centres are considered. The results show that this cytochrome displays redox-Bohr effects in the physiological pH range and that electrostatic effects dominate the macroscopic interactions between the acid-base and redox centres.

KW - Desulfovibrio desulfuricans

KW - Electrostatic models

KW - Energy transduction

KW - Nine-haem cytochome c

KW - Redox titrations

KW - Redox-Bohr effect

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JO - Inorganica Chimica Acta

JF - Inorganica Chimica Acta

ER -

Redox-Bohr effect in the nine haem cytochrome from Desulfovibrio desulfuricans 27774

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Keywords

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