TY - JOUR
T1 - Red disperse dyes (DR 60, DR 73 and DR 78) at environmentally realistic concentrations impact biochemical profile of early life stages of zebrafish (Danio rerio)
AU - Meireles, Gabriela
AU - Daam, Michiel A.
AU - Sanches, Ana Letícia Madeira
AU - Zanoni, Maria V. B.
AU - Soares, Amadeu M. V. M.
AU - Gravato, Carlos
AU - Oliveira, Danielle P. de
N1 - info:eu-repo/grantAgreement/FCT/5876/147274/PT#
info:eu-repo/grantAgreement/FCT/5876/147273/PT#
This work was supported by National Institute of Science and Technology for Detection, Toxicological Evaluation and Removal of Emerging and Radioactive Contaminants (INCT-DATREM); Sao Paulo Research Foundation (FAPESP - grant number 2016/03281-9). Scholarships were provided by National Council for Scientific and Technological Development (CNPq - grant number: 142379/2014-0), Coordination for the Improvement of Higher Education Personnel (CAPES - grant number 88881.134597/2016-01) through a doctoral grant for Gabriela Meireles and Sao Paulo Research Foundation through a doctoral grant for Ana Leticia Madeira Sanches (FAPESP - grant number 2017/05543-3 and 2014/14621-0). Special Visiting Researcher program (MEC/MCTI/CAPES/CNPq/FAPs reference 402392/2013-2) and Portuguese government (FCT) through a postdoc grant for Michiel A. Daam (SFRH/BPD/109199/2015) and the research unit UID/AMB/04085/2013 (CENSE). We also acknowledge FCT for Carlos Gravato contract (IF/01401/2014) and the funding support to CESAM (UID/AMB/50017) and co-funding by FEDER, within the PT2020 Partnership Agreement and Compete 2020.
PY - 2018/8/25
Y1 - 2018/8/25
N2 - Dyes have been used for more than twenty thousand years in textile, pharmaceutical, food, cosmetic, and photographic industries, among others. Despite their importance in these applications, dyes can be toxic and resistant to many degradation processes used in wastewater treatment plants. Although a large number of dyes have been released in the environments in high amounts, studies into the environmental toxicity of these substances are still scarce. The aim of this study was to evaluate the potential toxic effects of textile dyes Disperse Red 60, Disperse Red 73 and Disperse Red 78 in zebrafish early life stages. To this end, biochemical biomarkers were selected to evaluate non-enzimatic antioxidant (Total Glutathione), antioxidant enzymes (Glutathione S-transferase and Catalase), oxidative stress (lipid peroxidation), neurotransmission (acetylcholinesterase) and energetic metabolism parameters (energy available and energy consumed) after 96 h exposure to these dyes. Our results demonstrated that these disperse dyes induce biochemical alterations in zebrafish embryos at environmental realistic concentrations and that the discharge of these disperse dyes into water bodies should be carefully evaluated. The selected biomarkers were sensitive as early-warning endpoints of disperse dyes toxicity on zebrafish embryos. Implications for risk assessment and indications for future research are discussed.
AB - Dyes have been used for more than twenty thousand years in textile, pharmaceutical, food, cosmetic, and photographic industries, among others. Despite their importance in these applications, dyes can be toxic and resistant to many degradation processes used in wastewater treatment plants. Although a large number of dyes have been released in the environments in high amounts, studies into the environmental toxicity of these substances are still scarce. The aim of this study was to evaluate the potential toxic effects of textile dyes Disperse Red 60, Disperse Red 73 and Disperse Red 78 in zebrafish early life stages. To this end, biochemical biomarkers were selected to evaluate non-enzimatic antioxidant (Total Glutathione), antioxidant enzymes (Glutathione S-transferase and Catalase), oxidative stress (lipid peroxidation), neurotransmission (acetylcholinesterase) and energetic metabolism parameters (energy available and energy consumed) after 96 h exposure to these dyes. Our results demonstrated that these disperse dyes induce biochemical alterations in zebrafish embryos at environmental realistic concentrations and that the discharge of these disperse dyes into water bodies should be carefully evaluated. The selected biomarkers were sensitive as early-warning endpoints of disperse dyes toxicity on zebrafish embryos. Implications for risk assessment and indications for future research are discussed.
KW - Cellular energy allocation
KW - Neurotoxicity
KW - Oxidative stress
KW - Textile dyes
UR - http://www.scopus.com/inward/record.url?scp=85050114255&partnerID=8YFLogxK
U2 - 10.1016/j.cbi.2018.07.007
DO - 10.1016/j.cbi.2018.07.007
M3 - Article
C2 - 30006001
AN - SCOPUS:85050114255
SN - 0009-2797
VL - 292
SP - 94
EP - 100
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
ER -