Red blood cells in clinical proteomics: Methods in Molecular Biology

A.S. Carvalho, M.S. Rodriguez, R. Matthiesen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Red blood cells (RBCs) are known for their role in oxygen and carbon dioxide transport. The main function of RBCs is directly linked to many diseases that cause low oxygen levels in tissues such as congenital heart disease in adults, chronic obstructive pulmonary disease, sleep apnea, sickle cell disease, etc. Red blood cells are a direct target for a number of parasitic diseases such as malaria (Plasmodium) and similar parasites of the phylum Apicomplexa (Toxoplasma, Theileria, Eimeria, Babesia, and Cryptosporidium). RBC membrane components, in particular, are suitable targets for the discovery of drugs against parasite interaction. There is also evidence that RBCs release growth and survival factors, thereby linking RBCs with cancer. RBCs are abundant and travel throughout the body; consequently changes in RBC proteome potentially reflect other diseases as well. This chapter describes erythrocyte isolation from blood and its fractionation into RBC membrane and soluble cytosolic fractions. Alternative procedures for mass spectrometry analysis of RBC membrane proteome will be presented. © 2017, Springer Science+Business Media LLC.
Original languageEnglish
Title of host publicationRed blood cells in clinical proteomics: Methods in Molecular Biology
PublisherHumana Press Inc
Pages173-181
Number of pages9
Volume1619
ISBN (Print)10643745 (ISSN)
DOIs
Publication statusPublished - 2017

Keywords

  • Infection
  • Mass spectrometry
  • Membranar proteins
  • Proteases
  • Proteome
  • Red blood cell

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