TY - JOUR
T1 - Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil
AU - Martino, Lucrezia
AU - Cruz, Madalena V.
AU - Scoma, Alberto
AU - Freitas, Filomena
AU - Bertin, Lorenzo
AU - Scandola, Mariastella
AU - Reis, Maria A M
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Used cooking oil (UCO) was employed as the sole carbon source for the production of polyhydroxybutyrate (PHB) by cultivation in batch mode of Cupriavidus necator DSM 428. The produced biomass was used for extraction of the PHB granules with a solvent-free approach using sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and the enzyme Alcalase in an aqueous medium. The recovered PHB granules showed a degree of purity higher than 90% and no crystallization (i.e., granules were recovered in their 'native' amorphous state) as demonstrated by wide angle X-ray diffraction (WAXS). Granules were characterized according to their thermal properties and stability by differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Results show that UCO can be used as a renewable resource to produce amorphous PHB granules with excellent properties in a biocompatible manner.
AB - Used cooking oil (UCO) was employed as the sole carbon source for the production of polyhydroxybutyrate (PHB) by cultivation in batch mode of Cupriavidus necator DSM 428. The produced biomass was used for extraction of the PHB granules with a solvent-free approach using sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA), and the enzyme Alcalase in an aqueous medium. The recovered PHB granules showed a degree of purity higher than 90% and no crystallization (i.e., granules were recovered in their 'native' amorphous state) as demonstrated by wide angle X-ray diffraction (WAXS). Granules were characterized according to their thermal properties and stability by differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Results show that UCO can be used as a renewable resource to produce amorphous PHB granules with excellent properties in a biocompatible manner.
KW - Biocompatible extraction
KW - Biowaste
KW - Native amorphous granule
KW - Polyhydroxyalkanoate (PHA)
KW - Spent oil
KW - Used cooking oil (UCO)
UR - http://www.scopus.com/inward/record.url?scp=84911147421&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2014.04.016
DO - 10.1016/j.ijbiomac.2014.04.016
M3 - Article
C2 - 24751509
AN - SCOPUS:84911147421
SN - 0141-8130
VL - 71
SP - 117
EP - 123
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - SI
ER -
