Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes

Oscar Gutiérrez-Sanz, David Olea, Marcos Pita, Ana P. Batista, Alvaro Alonso, Manuela Alexandra Pereira, Marisela Vélez, Antonio L. De Lacey

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

For the first time, respiratory complex I has been reconstituted on an electrode preserving its structure and activity. Respiratory complex I is a membrane-bound enzyme that has an essential function in cellular energy production. It couples NADH:quinone oxidoreduction to translocation of ions across the cellular (in prokaryotes) or mitochondrial membranes. Therefore, complex I contributes to the establishment and maintenance of the transmembrane difference of electrochemical potential required for adenosine triphosphate synthesis, transport, and motility. Our new strategy has been applied for reconstituting the bacterial complex I from Rhodothermus marinus onto a biomimetic membrane supported on gold electrodes modified with a thiol self-assembled monolayer (SAM). Atomic force microscopy and faradaic impedance measurements give evidence of the biomimetic construction, whereas electrochemical measurements show its functionality. Both electron transfer and proton translocation by respiratory complex I were monitored, simulating in vivo conditions.

Original languageEnglish
Pages (from-to)9007-9015
Number of pages9
JournalLangmuir
Volume30
Issue number29
DOIs
Publication statusPublished - 29 Jul 2014

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