Abstract
| Original language | Unknown |
|---|---|
| Pages (from-to) | 44-9 |
| Journal | Biosensors & Bioelectronics |
| Volume | 28 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 15 Nov 2011 |
Cite this
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Real-time monitoring of PCR amplification of proto-oncogene c-MYC using a Ta₂O₅ electrolyte-insulator-semiconductor sensor. / Branquinho, Rita; Veigas, Bruno; Vaz Pinto, Joana; Martins, Rodrigo Ferrão de Paiva; Fortunato, Elvira Maria Correia; Baptista, Pedro Miguel Ribeiro Viana.
In: Biosensors & Bioelectronics, Vol. 28, No. 1, 15.11.2011, p. 44-9.Research output: Contribution to journal › Article
TY - JOUR
T1 - Real-time monitoring of PCR amplification of proto-oncogene c-MYC using a Ta₂O₅ electrolyte-insulator-semiconductor sensor.
AU - Branquinho, Rita
AU - Veigas, Bruno
AU - Vaz Pinto, Joana
AU - Martins, Rodrigo Ferrão de Paiva
AU - Fortunato, Elvira Maria Correia
AU - Baptista, Pedro Miguel Ribeiro Viana
PY - 2011/11/15
Y1 - 2011/11/15
N2 - We present a new approach for real-time monitoring of PCR amplification of a specific sequence from the human c-MYC proto-oncogene using a Ta(2)O(5) electrolyte-insulator-semiconductor (EIS) sensor. The response of the fabricated EIS sensor to cycle DNA amplification was evaluated and compared to standard SYBR-green fluorescence incorporation, showing it was possible to detect DNA concentration variations with 30 mV/μM sensitivity. The sensor's response was then optimized to follow in real-time the PCR amplification of c-MYC sequence from a genomic DNA sample attaining an amplification profile comparable to that of a standard real-time PCR. Owing to the small size, ease of fabrication and low-cost, the developed Ta(2)O(5) sensor may be incorporated onto a microfluidic device and then used for real-time PCR. Our approach may circumvent the practical and economical obstacles posed by current platforms that require an external fluorescence detector difficult to miniaturize and incorporate into a lab-on-chip system.
AB - We present a new approach for real-time monitoring of PCR amplification of a specific sequence from the human c-MYC proto-oncogene using a Ta(2)O(5) electrolyte-insulator-semiconductor (EIS) sensor. The response of the fabricated EIS sensor to cycle DNA amplification was evaluated and compared to standard SYBR-green fluorescence incorporation, showing it was possible to detect DNA concentration variations with 30 mV/μM sensitivity. The sensor's response was then optimized to follow in real-time the PCR amplification of c-MYC sequence from a genomic DNA sample attaining an amplification profile comparable to that of a standard real-time PCR. Owing to the small size, ease of fabrication and low-cost, the developed Ta(2)O(5) sensor may be incorporated onto a microfluidic device and then used for real-time PCR. Our approach may circumvent the practical and economical obstacles posed by current platforms that require an external fluorescence detector difficult to miniaturize and incorporate into a lab-on-chip system.
KW - EIS
KW - Real-time PCR
KW - Cancer
KW - Tantalum pentoxide
KW - DNA quantification
KW - Sensors/Biosensors
KW - Field effect based sensors
U2 - 10.1016/j.bios.2011.06.039
DO - 10.1016/j.bios.2011.06.039
M3 - Article
VL - 28
SP - 44
EP - 49
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
IS - 1
ER -