Rapid differentiation of species of Botryosphaeriaceae by PCR fingerprinting

Alan John Lander Phillips

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The fingerprinting methods referred to as MSP-PCR (microsatellite-primed polymerase chain reaction) and rep-PCR (repetitive-sequence-based polymerase chain reaction) were used to discriminate between species of Botryosphaeriaceae (Fungi, Ascomycota). Several primers were tested with both methods and each primer enabled clear differentiation of all the species tested. Cluster analysis of banding patterns of the isolates corresponded well with known species delineations based on morphology and phylogenetic analysis. The methods described in this paper provide simple and rapid procedures that can be used for routine differentiation of Botryosphaeriaceae isolates at the species level. The two methods are also useful for studying intraspecific variability. (c) 2006 Elsevier Masson SAS. All rights reserved.
Original languageUnknown
Pages (from-to)112-121
JournalResearch In Microbiology
Volume158
Issue number2
DOIs
Publication statusPublished - 1 Jan 2007

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