Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip

Lucia S. Cheung, Xi Wei, Diogo Martins, Yong Ak Song

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.

Original languageEnglish
Article number014104
JournalBiomicrofluidics
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2018

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