Radiation damage on Langmuir monolayers of the anionic 1.2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt)(DPPG) phospholipid at the air-DNA solution interface

Paulo J. Gomes, Amélia M P S Gonçalves Da Silva, Paulo A. Ribeiro, Osvaldo N. Oliveira, Maria Raposo

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The resilience of cells to ultraviolet (UV) irradiation is probably associated with the effects induced in biological molecules such as DNA and in the cell membrane. In this study, we investigated UV damage to the anionic 1.2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DPPG) phospholipid, which is an important component of cell membranes. In films cast from DPPG emulsions, UV irradiation induced cleavage of C - O, C = O and - PO2 - bonds, while in Langmuir monolayers at the air/water interface representing the cell membrane this irradiation caused the monolayer stability to decrease. When DNA was present in the subphase, however, the effects from UV irradiation were smaller, since the ionic products from degradation of either DPPG or DNA stabilize the intact DPPG molecules. This mechanism may explain why UV irradiation does not cause immediate cell collapse, thus providing time for the cellular machinery to repair elements damaged by UV.

Original languageEnglish
Article number5729
Pages (from-to)576-579
Number of pages4
JournalMATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume58
DOIs
Publication statusPublished - 1 Jan 2016

Keywords

  • DNA
  • DPPG
  • Langmuir films
  • Radiation damage
  • UV radiation

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