Rab27a-mediated protease release regulates neutrophil recruitment by allowing uropod detachment

Rajesh K Singh, Wenjia Liao, Dhani Tracey-White, Chiara Recchi, Tanya Tolmachova, Sara M Rankin, Alistair N Hume, Miguel C Seabra

Research output: Contribution to journalArticle

15 Citations (Scopus)
2 Downloads (Pure)

Abstract

Neutrophil migration is vital for immunity and precedes effector functions such as pathogen killing. Here, we report that this process is regulated by the Rab27a GTPase, a protein known to control granule exocytosis. Rab27a-deficient (Rab27a KO) neutrophils exhibit migration defects in vitro and in vivo, and live-cell microscopy suggests that delayed uropod detachment causes the migratory defect. Surface expression of CD11b, a key adhesion molecule, is increased in chemokine-stimulated Rab27a KO neutrophils compared with the control, suggesting a turnover delay caused by a defect in elastase secretion from azurophilic granules at the rear of bone marrow polymorphonuclear leukocytes (BM-PMNs). We suggest that Rab27a-dependent protease secretion regulates neutrophil migration through proteolysis-dependent de-adhesion of uropods, a mechanism that could be conserved in cell migration and invasion.
Original languageEnglish
Pages (from-to)1652-1656
Number of pages5
JournalJournal Of Cell Science
Volume125
Issue numberPt 7
DOIs
Publication statusPublished - 1 Apr 2012

Keywords

  • Rab27a
  • Chemotaxis
  • Cell migration
  • Neutrophil
  • Uropod

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