Quantitative proteomic analysis of ibuprofen-degrading Patulibacter sp strain I11

Bárbara Almeida, Henrik Kjeldal, Ihab Bishara Lolas, Anders Dahl Knudsen, Gilda Carvalho, Kåre Lehmann Nielsen, Maria Teresa Barreto Goulão Crespo, Allan Stensballe, Jeppe Lund Nielsen

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Ibuprofen is the third most consumed pharmaceutical drug in the world. Several isolates have been shown to degrade ibuprofen, but very little is known about the biochemistry of this process. This study investigates the degradation of ibuprofen by Patulibacter sp. strain I11 by quantitative proteomics using a metabolic labelling strategy. The whole-genome of Patulibacter sp. strain I11 was sequenced to provide a species-specific protein platform for optimal protein identification. The bacterial proteomes of actively ibuprofen-degrading cells and cells grown in the absence of ibuprofen was identified and quantified by gel based shotgun-proteomics. In total 251 unique proteins were quantitated using this approach. Biological process and pathway analysis indicated a number of proteins that were up-regulated in response to active degradation of ibuprofen, some of them are known to be involved in the degradation of aromatic compounds. Data analysis revealed that several of these proteins are likely involved in ibuprofen degradation by Patulibacter sp. strain I11.
Original languageEnglish
Pages (from-to)615-630
Issue number5
Publication statusPublished - Sep 2013


  • Aromatic compounds
  • Degradation
  • Ibuprofen
  • Patulibacter sp.
  • Quantitative proteomics


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