Quantification of the arylesterase activity of paraoxonase-1 in human blood

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Paraoxonase-1 (PON1) is known as a free-radical scavenging system associated with circulating serum high-density lipoprotein (HDL). PON1 catalyzes the hydrolysis of multiple compounds such as arylesters, lactones and hydroperoxides. The arylesterase (AREase) activity of PON1 is involved in the detoxification of lipid peroxides, which are related to several clinical conditions. Therefore, the possibility of measuring the AREase activity in routine clinical studies would be advantageous. The AREase activity was obtained by monitoring the formation of acetic acid, upon the hydrolysis of phenyl acetate, using 10 mu L of sample. The method accuracy was higher than 90\% and intra-assay and inter-assay precisions were 96\% and 95\%, respectively. The method validation supported that this analytical procedure is suitable for use in human serum and heparinized plasma samples, while ethylenediaminetetra-acetic acid (EDTA)containing samples should be avoided. The methodology herein described constitutes an easy, fast and reliable method for assessing the AREase activity of PON1. This method can be easily implemented as a clinical analytical tool and is also suitable for research purposes.
Original languageEnglish
Pages (from-to)289-294
Number of pages5
JournalAnalytical Methods
Issue number1
Publication statusPublished - 2014


Dive into the research topics of 'Quantification of the arylesterase activity of paraoxonase-1 in human blood'. Together they form a unique fingerprint.

Cite this