TY - JOUR
T1 - Quality control of next-generation sequencing-based HIV-1 drug resistance data in clinical laboratory information systems framework
AU - Capina, Rupert
AU - Li, Katherine
AU - Kearney, Levon
AU - Vandamme, Anne Mieke
AU - Harrigan, P. Richard
AU - Van Laethem, Kristel
N1 - Funding Information:
We acknowledge the Fonds voor Wetenschappelijk Onderzoek Vlaanderen (FWO) (G.0B23.17N) for covering publications costs.
Publisher Copyright:
© 2020 by the authors.
PY - 2020/6
Y1 - 2020/6
N2 - Next-generation sequencing (NGS) in HIV drug resistance (HIVDR) testing has the potential to improve both clinical and public health settings, however it challenges the normal operations of quality management systems to be more flexible due to its complexity, massive data generation, and rapidly evolving protocols. While guidelines for quality management inNGSdata have previously been outlined, little guidance has been implemented for NGS-based HIVDR testing. This document summarizes quality control procedures for NGS-based HIVDR testing laboratories using a laboratory information systems (LIS) framework. Here, we focus in particular on the quality control measures applied on the final sequencing product aligned with the recommendations from theWorld Health Organization HIV Drug Resistance Laboratory Network.
AB - Next-generation sequencing (NGS) in HIV drug resistance (HIVDR) testing has the potential to improve both clinical and public health settings, however it challenges the normal operations of quality management systems to be more flexible due to its complexity, massive data generation, and rapidly evolving protocols. While guidelines for quality management inNGSdata have previously been outlined, little guidance has been implemented for NGS-based HIVDR testing. This document summarizes quality control procedures for NGS-based HIVDR testing laboratories using a laboratory information systems (LIS) framework. Here, we focus in particular on the quality control measures applied on the final sequencing product aligned with the recommendations from theWorld Health Organization HIV Drug Resistance Laboratory Network.
KW - HIV-1 drug resistance
KW - Laboratory information systems
KW - Next-generation sequencing
KW - Quality control
KW - Quality management
UR - http://www.scopus.com/inward/record.url?scp=85086686572&partnerID=8YFLogxK
U2 - 10.3390/v12060645
DO - 10.3390/v12060645
M3 - Review article
C2 - 32545906
AN - SCOPUS:85086686572
SN - 1999-4915
VL - 12
JO - Viruses
JF - Viruses
IS - 6
M1 - 645
ER -
