Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774

Olga Yu Gavel; Anna V. Kladova; Sergey A. Bursakov; João M. Dias; Susana Texeira; Valery L. Shnyrov; José J. G. Moura; Isabel Moura; Maria J. Romão; José Trincão

doi:10.1107/S1744309108008816

Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774

Olga Yu Gavel, Anna V. Kladova, Sergey A. Bursakov, João M. Dias, Susana Texeira, Valery L. Shnyrov, José J. G. Moura, Isabel Moura, Maria J. Romão, José Trincão

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

36 Downloads (Pure)

Abstract

Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP:sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 Å resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes.

Original language	English
Pages (from-to)	593-595
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	64
Issue number	7
DOIs	https://doi.org/10.1107/S1744309108008816
Publication status	Published - 1 Jan 2008

Keywords

ATP sulfurylases
Cobalt
Sulfate-reducing bacteria
Zinc

Access to Document

10.1107/S1744309108008816Licence: CC BY

f-64-00593Final published version, 338 KBLicence: CC BY

Cite this

Gavel, O. Y., Kladova, A. V., Bursakov, S. A., Dias, J. M., Texeira, S., Shnyrov, V. L., Moura, J. J. G., Moura, I., Romão, M. J., & Trincão, J. (2008). Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 64(7), 593-595. https://doi.org/10.1107/S1744309108008816

Gavel, Olga Yu ; Kladova, Anna V. ; Bursakov, Sergey A. et al. / Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2008 ; Vol. 64, No. 7. pp. 593-595.

@article{4066e9ff328f4a8fb28fa922f976111a,

title = "Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774",

abstract = "Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP:sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 {\AA} resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes.",

keywords = "ATP sulfurylases, Cobalt, Sulfate-reducing bacteria, Zinc",

author = "Gavel, {Olga Yu} and Kladova, {Anna V.} and Bursakov, {Sergey A.} and Dias, {Jo{\~a}o M.} and Susana Texeira and Shnyrov, {Valery L.} and Moura, {Jos{\'e} J. G.} and Isabel Moura and Rom{\~a}o, {Maria J.} and Jos{\'e} Trinc{\~a}o",

year = "2008",

month = jan,

day = "1",

doi = "10.1107/S1744309108008816",

language = "English",

volume = "64",

pages = "593--595",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Blackwell Publishing Ltd",

number = "7",

}

Gavel, OY , Kladova, AV , Bursakov, SA , Dias, JM , Texeira, S, Shnyrov, VL, Moura, JJG , Moura, I , Romão, MJ & Trincão, J 2008, 'Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 64, no. 7, pp. 593-595. https://doi.org/10.1107/S1744309108008816

Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774. / Gavel, Olga Yu ; Kladova, Anna V.; Bursakov, Sergey A. et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 64, No. 7, 01.01.2008, p. 593-595.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774

AU - Gavel, Olga Yu

AU - Kladova, Anna V.

AU - Bursakov, Sergey A.

AU - Dias, João M.

AU - Texeira, Susana

AU - Shnyrov, Valery L.

AU - Moura, José J. G.

AU - Moura, Isabel

AU - Romão, Maria J.

AU - Trincão, José

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP:sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 Å resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes.

AB - Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP:sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 Å resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical studies of this protein indicated the presence of a homotrimer in solution, a dimer was found in the asymmetric unit. Elucidation of this structure will permit a better understanding of the role of the metal in the activity and stability of this family of enzymes.

KW - ATP sulfurylases

KW - Cobalt

KW - Sulfate-reducing bacteria

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=46949100709&partnerID=8YFLogxK

U2 - 10.1107/S1744309108008816

DO - 10.1107/S1744309108008816

M3 - Article

C2 - 18607083

AN - SCOPUS:46949100709

SN - 1744-3091

VL - 64

SP - 593

EP - 595

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 7

ER -

Gavel OY , Kladova AV , Bursakov SA , Dias JM , Texeira S, Shnyrov VL et al. Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774. Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2008 Jan 1;64(7):593-595. doi: 10.1107/S1744309108008816

Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this