Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

Ilda Santos Sanches

doi:10.1007/978-1-4939-2004-4_23

Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.

Original language	English
Title of host publication	Veterinary Infection Biology
Subtitle of host publication	Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology
Editors	M. Cunha, J. Inácio
Place of Publication	New York
Publisher	Springer
Pages	323-334
Volume	1247
ISBN (Electronic)	978-149392003-7
ISBN (Print)	978-1-4939-2004-4
DOIs	https://doi.org/10.1007/978-1-4939-2004-4_23
Publication status	Published - Nov 2015

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
ISSN (Print)	1064-3745

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10.1007/978-1-4939-2004-4_23

http://link.springer.com/protocol/10.1007%2F978-1-4939-2004-4_23

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Santos Sanches, I. (2015). Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. In M. Cunha, & J. Inácio (Eds.), Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology (Vol. 1247, pp. 323-334). (Methods in Molecular Biology). Springer. https://doi.org/10.1007/978-1-4939-2004-4_23

Santos Sanches, Ilda. / Pulsed-Field Gel Electrophoresis (PFGE) : Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. editor / M. Cunha ; J. Inácio. Vol. 1247 New York : Springer, 2015. pp. 323-334 (Methods in Molecular Biology).

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title = "Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..",

abstract = "Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.",

author = "{Santos Sanches}, Ilda",

year = "2015",

month = nov,

doi = "10.1007/978-1-4939-2004-4_23",

language = "English",

isbn = "978-1-4939-2004-4",

volume = "1247",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "323--334",

editor = "M. Cunha and J. In{\'a}cio",

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Santos Sanches, I 2015, Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. in M Cunha & J Inácio (eds), Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. vol. 1247, Methods in Molecular Biology, Springer, New York, pp. 323-334. https://doi.org/10.1007/978-1-4939-2004-4_23

Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. / Santos Sanches, Ilda.
Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. ed. / M. Cunha; J. Inácio. Vol. 1247 New York: Springer, 2015. p. 323-334 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Pulsed-Field Gel Electrophoresis (PFGE)

T2 - Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

AU - Santos Sanches, Ilda

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Y1 - 2015/11

N2 - Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.

AB - Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.

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DO - 10.1007/978-1-4939-2004-4_23

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SN - 978-1-4939-2004-4

VL - 1247

T3 - Methods in Molecular Biology

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BT - Veterinary Infection Biology

A2 - Cunha, M.

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Santos Sanches I. Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. In Cunha M, Inácio J, editors, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. Vol. 1247. New York: Springer. 2015. p. 323-334. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-2004-4_23

Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

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