Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.
Original languageEnglish
Title of host publicationVeterinary Infection Biology
Subtitle of host publicationMolecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology
EditorsM. Cunha, J. Inácio
Place of PublicationNew York
PublisherSpringer
Pages323-334
Volume1247
ISBN (Electronic)978-149392003-7
ISBN (Print)978-1-4939-2004-4
DOIs
Publication statusPublished - Nov 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745

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