Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.
Original languageEnglish
Title of host publicationVeterinary Infection Biology
Subtitle of host publicationMolecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology
EditorsM. Cunha, J. Inácio
Place of PublicationNew York
PublisherSpringer
Pages323-334
Volume1247
ISBN (Electronic)978-149392003-7
ISBN (Print)978-1-4939-2004-4
DOIs
Publication statusPublished - Nov 2015

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745

Fingerprint

Bovine Mastitis
Pulsed Field Gel Electrophoresis
Streptococcus
Streptococcus agalactiae
Population
Dairying
Agar Gel Electrophoresis
Mastitis
Epidemiologic Studies
Milk
DNA

Cite this

Santos Sanches, I. (2015). Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. In M. Cunha, & J. Inácio (Eds.), Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology (Vol. 1247, pp. 323-334). (Methods in Molecular Biology). New York: Springer. https://doi.org/10.1007/978-1-4939-2004-4_23
Santos Sanches, Ilda. / Pulsed-Field Gel Electrophoresis (PFGE) : Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. editor / M. Cunha ; J. Inácio. Vol. 1247 New York : Springer, 2015. pp. 323-334 (Methods in Molecular Biology).
@inbook{67ef2f2141c64e3e86e20e763eec81ce,
title = "Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..",
abstract = "Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.",
author = "{Santos Sanches}, Ilda",
year = "2015",
month = "11",
doi = "10.1007/978-1-4939-2004-4_23",
language = "English",
isbn = "978-1-4939-2004-4",
volume = "1247",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "323--334",
editor = "M. Cunha and J. In{\'a}cio",
booktitle = "Veterinary Infection Biology",

}

Santos Sanches, I 2015, Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. in M Cunha & J Inácio (eds), Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. vol. 1247, Methods in Molecular Biology, Springer, New York, pp. 323-334. https://doi.org/10.1007/978-1-4939-2004-4_23

Pulsed-Field Gel Electrophoresis (PFGE) : Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. / Santos Sanches, Ilda.

Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. ed. / M. Cunha; J. Inácio. Vol. 1247 New York : Springer, 2015. p. 323-334 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Pulsed-Field Gel Electrophoresis (PFGE)

T2 - Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci..

AU - Santos Sanches, Ilda

PY - 2015/11

Y1 - 2015/11

N2 - Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.

AB - Pulsed-field gel electrophoresis (PFGE) separates large DNA molecules by the use of an alternating electrical field, such that greater size resolution can be obtained when compared to normal agarose gel electrophoresis. PFGE is often employed to track pathogens and is a valuable typing scheme to detect and differentiate strains. Particularly, the contour-clamped homogeneous electric field (CHEF) PFGE system is considered to be the gold standard for use in epidemiological studies of many bacterial pathogens. Here we describe a PFGE protocol that was applicable to the study of bovine streptococci, namely, Streptococcus agalactiae (group B Streptococcus, GBS), Streptococcus dysgalactiae subsp. dysgalactiae (group C Streptococcus, GCS), and Streptococcus uberis-which are relevant pathogens causing mastitis, a highly prevalent and costly disease in dairy industry due to antibiotherapy and loss in milk production.

U2 - 10.1007/978-1-4939-2004-4_23

DO - 10.1007/978-1-4939-2004-4_23

M3 - Chapter

SN - 978-1-4939-2004-4

VL - 1247

T3 - Methods in Molecular Biology

SP - 323

EP - 334

BT - Veterinary Infection Biology

A2 - Cunha, M.

A2 - Inácio, J.

PB - Springer

CY - New York

ER -

Santos Sanches I. Pulsed-Field Gel Electrophoresis (PFGE): Application in Population Structure Studies of Bovine Mastitis-Causing Streptococci.. In Cunha M, Inácio J, editors, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies, Methods in Molecular Biology. Vol. 1247. New York: Springer. 2015. p. 323-334. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2004-4_23