TY - JOUR
T1 - Pseudomonas chlororaphis as a multiproduct platform: Conversion of glycerol into high-value biopolymers and phenazines
AU - de Meneses, Liane Pereira
AU - Pereira, João Ricardo
AU - Sevrin, Chantal
AU - Grandfils, Christian
AU - Paiva, Alexandre
AU - Reis, Maria A. M.
AU - Freitas, Filomena
N1 - Applied Molecular Biosciences Unit (UCIBIO) and the Associated Laboratory for Green Chemistry (LAQV), which are financed by national funds from FCT/MCTES (UID/Multi/04378/2019, UID/QUI/50006/2019);
project Des-Zyme (PTDC/BBBEBB/1676/2014).
PY - 2020/3/25
Y1 - 2020/3/25
N2 - Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated using glycerol as the sole carbon source for the simultaneous production of medium-chain length polyhydroxyalkanoates (mcl-PHA), extracellular polysaccharide (EPS) and phenazines. A maximum cell dry mass of 11.79 g/L was achieved with a mcl-PHA content of 19 wt%, corresponding to a polymer concentration of 2.23 g/L. A considerably higher EPS production, 6.10 g/L, was attained. Phenazines synthesis was evidenced by the bright orange coloration developed by the culture during the cell growth phase. The mcl-PHA produced by P. chlororaphis was composed mainly of 3-hydroxydecanoate (50 wt%) with lower amounts of 3-hydroxyoctanoate (17 wt%), 3-hydroxytetradecanoate (17 wt%), 3-hydroxydodecanoate (13 wt%) and 3-hydroxyhexanoate (3 wt%). This PHA showed unique thermal features being highly amorphous, with a degree of crystallinity of 27% and a low melting temperature (45.0 °C). The secreted EPS was mostly composed of glucose, glucosamine, rhamnose and mannose, with smaller amounts of three other unidentified monomers. Although the bioprocess can be improved further to define the optimal conditions to produce each bioproduct (mcl-PHA, EPS or phenazines), this study has demonstrated for the first time the ability of P. chlororaphis to simultaneously produce three high-value products from a single substrate.
AB - Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated using glycerol as the sole carbon source for the simultaneous production of medium-chain length polyhydroxyalkanoates (mcl-PHA), extracellular polysaccharide (EPS) and phenazines. A maximum cell dry mass of 11.79 g/L was achieved with a mcl-PHA content of 19 wt%, corresponding to a polymer concentration of 2.23 g/L. A considerably higher EPS production, 6.10 g/L, was attained. Phenazines synthesis was evidenced by the bright orange coloration developed by the culture during the cell growth phase. The mcl-PHA produced by P. chlororaphis was composed mainly of 3-hydroxydecanoate (50 wt%) with lower amounts of 3-hydroxyoctanoate (17 wt%), 3-hydroxytetradecanoate (17 wt%), 3-hydroxydodecanoate (13 wt%) and 3-hydroxyhexanoate (3 wt%). This PHA showed unique thermal features being highly amorphous, with a degree of crystallinity of 27% and a low melting temperature (45.0 °C). The secreted EPS was mostly composed of glucose, glucosamine, rhamnose and mannose, with smaller amounts of three other unidentified monomers. Although the bioprocess can be improved further to define the optimal conditions to produce each bioproduct (mcl-PHA, EPS or phenazines), this study has demonstrated for the first time the ability of P. chlororaphis to simultaneously produce three high-value products from a single substrate.
KW - Co-production
KW - Extracellular polysaccharide
KW - Medium-chain-length polyhydroxyalkanoate
KW - Phenazines
KW - Pseudomonas chlororaphis
UR - http://www.scopus.com/inward/record.url?scp=85073690756&partnerID=8YFLogxK
U2 - 10.1016/j.nbt.2019.10.002
DO - 10.1016/j.nbt.2019.10.002
M3 - Article
C2 - 31605767
AN - SCOPUS:85073690756
SN - 1871-6784
VL - 55
SP - 84
EP - 90
JO - New Biotechnology
JF - New Biotechnology
ER -