Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calciumregulation proteins and acute inflammatory response

E. Oliveira; J.E. Araújo; S. Gómez-Meire; C. Lodeiro; Cristina Perez-Melon; Elena Iglesias-Lamas; Alfonso Otero-Glez; J.L. Capelo; H.M. Santos

doi:10.1186/1559-0275-11-17

Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calciumregulation proteins and acute inflammatory response

E. Oliveira, J.E. Araújo, S. Gómez-Meire, C. Lodeiro, Cristina Perez-Melon, Elena Iglesias-Lamas, Alfonso Otero-Glez, J.L. Capelo, H.M. Santos

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies. © 2014 Oliveira et al.; licensee BioMed Central Ltd.

Original language	English
Journal	Clinical Proteomics
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/1559-0275-11-17
Publication status	Published - 2014

Keywords

2D-gel electrophoresis
Peritoneal dialysis effluent
Protein identification
Proteomics
albumin
alpha 1 antitrypsin
apolipoprotein
apolipoprotein 4
apolipoprotein A1
beta2 glycoprotein
calcium
ceruloplasmin
complement factor D
cystathionine beta synthase
dithiothreitol
fetuin A
fibrinogen
fibrinogen beta chain
fibrinogen gamma chain
glycoprotein
haptoglobin
immunoglobulin G
immunoglobulin G gamma 1 chain C
immunoglobulin G kappa chain C region
orosomucoid
phosphoprotein phosphatase
protein AMBP
protein phosphatase 1D
proteome
retinol binding protein 4
Serotransferrin
transferrin
unclassified drug
zinc alpha 2 glycoprotein
adult
aged
article
calcium metabolism
clinical article
dialysate
female
human
inflammation
kidney injury
male
peritoneal dialysis
priority journal
protein analysis
protein expression
proteomics
regulatory mechanism
soft tissue calcification
tandem mass spectrometry

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title = "Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calciumregulation proteins and acute inflammatory response",

abstract = "Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies. {\textcopyright} 2014 Oliveira et al.; licensee BioMed Central Ltd.",

keywords = "2D-gel electrophoresis, Peritoneal dialysis effluent, Protein identification, Proteomics, albumin, alpha 1 antitrypsin, apolipoprotein, apolipoprotein 4, apolipoprotein A1, beta2 glycoprotein, calcium, ceruloplasmin, complement factor D, cystathionine beta synthase, dithiothreitol, fetuin A, fibrinogen, fibrinogen beta chain, fibrinogen gamma chain, glycoprotein, haptoglobin, immunoglobulin G, immunoglobulin G gamma 1 chain C, immunoglobulin G kappa chain C region, orosomucoid, phosphoprotein phosphatase, protein AMBP, protein phosphatase 1D, proteome, retinol binding protein 4, Serotransferrin, transferrin, unclassified drug, zinc alpha 2 glycoprotein, adult, aged, article, calcium metabolism, clinical article, dialysate, female, human, inflammation, kidney injury, male, peritoneal dialysis, priority journal, protein analysis, protein expression, proteomics, regulatory mechanism, soft tissue calcification, tandem mass spectrometry",

author = "E. Oliveira and J.E. Ara{\'u}jo and S. G{\'o}mez-Meire and C. Lodeiro and Cristina Perez-Melon and Elena Iglesias-Lamas and Alfonso Otero-Glez and J.L. Capelo and H.M. Santos",

year = "2014",

doi = "10.1186/1559-0275-11-17",

language = "English",

volume = "11",

journal = "Clinical Proteomics",

issn = "1542-6416",

publisher = "BioMed Central (BMC)",

number = "1",

}

TY - JOUR

T1 - Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calciumregulation proteins and acute inflammatory response

AU - Oliveira, E.

AU - Araújo, J.E.

AU - Gómez-Meire, S.

AU - Lodeiro, C.

AU - Perez-Melon, Cristina

AU - Iglesias-Lamas, Elena

AU - Otero-Glez, Alfonso

AU - Capelo, J.L.

AU - Santos, H.M.

PY - 2014

Y1 - 2014

N2 - Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies. © 2014 Oliveira et al.; licensee BioMed Central Ltd.

AB - Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies. © 2014 Oliveira et al.; licensee BioMed Central Ltd.

KW - 2D-gel electrophoresis

KW - Peritoneal dialysis effluent

KW - Protein identification

KW - Proteomics

KW - albumin

KW - alpha 1 antitrypsin

KW - apolipoprotein

KW - apolipoprotein 4

KW - apolipoprotein A1

KW - beta2 glycoprotein

KW - calcium

KW - ceruloplasmin

KW - complement factor D

KW - cystathionine beta synthase

KW - dithiothreitol

KW - fetuin A

KW - fibrinogen

KW - fibrinogen beta chain

KW - fibrinogen gamma chain

KW - glycoprotein

KW - haptoglobin

KW - immunoglobulin G

KW - immunoglobulin G gamma 1 chain C

KW - immunoglobulin G kappa chain C region

KW - orosomucoid

KW - phosphoprotein phosphatase

KW - protein AMBP

KW - protein phosphatase 1D

KW - proteome

KW - retinol binding protein 4

KW - Serotransferrin

KW - transferrin

KW - unclassified drug

KW - zinc alpha 2 glycoprotein

KW - adult

KW - aged

KW - article

KW - calcium metabolism

KW - clinical article

KW - dialysate

KW - female

KW - human

KW - inflammation

KW - kidney injury

KW - male

KW - peritoneal dialysis

KW - priority journal

KW - protein analysis

KW - protein expression

KW - proteomics

KW - regulatory mechanism

KW - soft tissue calcification

KW - tandem mass spectrometry

U2 - 10.1186/1559-0275-11-17

DO - 10.1186/1559-0275-11-17

M3 - Article

SN - 1542-6416

VL - 11

JO - Clinical Proteomics

JF - Clinical Proteomics

IS - 1

ER -

Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calciumregulation proteins and acute inflammatory response

Abstract

Keywords

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