Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calciumregulation proteins and acute inflammatory response

E. Oliveira, J.E. Araújo, S. Gómez-Meire, C. Lodeiro, Cristina Perez-Melon, Elena Iglesias-Lamas, Alfonso Otero-Glez, J.L. Capelo, H.M. Santos

Research output: Contribution to journalArticle

Abstract

Background: Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. Results: A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. Conclusions: Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies. © 2014 Oliveira et al.; licensee BioMed Central Ltd.
Original languageEnglish
JournalClinical Proteomics
Volume11
Issue number1
DOIs
Publication statusPublished - 2014

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Keywords

  • 2D-gel electrophoresis
  • Peritoneal dialysis effluent
  • Protein identification
  • Proteomics
  • albumin
  • alpha 1 antitrypsin
  • apolipoprotein
  • apolipoprotein 4
  • apolipoprotein A1
  • beta2 glycoprotein
  • calcium
  • ceruloplasmin
  • complement factor D
  • cystathionine beta synthase
  • dithiothreitol
  • fetuin A
  • fibrinogen
  • fibrinogen beta chain
  • fibrinogen gamma chain
  • glycoprotein
  • haptoglobin
  • immunoglobulin G
  • immunoglobulin G gamma 1 chain C
  • immunoglobulin G kappa chain C region
  • orosomucoid
  • phosphoprotein phosphatase
  • protein AMBP
  • protein phosphatase 1D
  • proteome
  • retinol binding protein 4
  • Serotransferrin
  • transferrin
  • unclassified drug
  • zinc alpha 2 glycoprotein
  • adult
  • aged
  • article
  • calcium metabolism
  • clinical article
  • dialysate
  • female
  • human
  • inflammation
  • kidney injury
  • male
  • peritoneal dialysis
  • priority journal
  • protein analysis
  • protein expression
  • proteomics
  • regulatory mechanism
  • soft tissue calcification
  • tandem mass spectrometry

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