Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response

Elisabete Oliveira; Jose E. Araujo; Silvana Gomez-Meire; Carlos Lodeiro; Cristina Perez-Melon; Elena Iglesias-Lamas; Alfonso Otero-Glez; José Luis Capelo; Hugo M. Santos

doi:10.1186/1559-0275-11-17

Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response

Elisabete Oliveira, Jose E. Araujo, Silvana Gomez-Meire, Carlos Lodeiro, Cristina Perez-Melon, Elena Iglesias-Lamas, Alfonso Otero-Glez, José Luis Capelo, Hugo M. Santos

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

BACKGROUND Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. RESULTS A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. CONCLUSIONS Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.

Original language	English
Pages (from-to)	17
Journal	Clinical Proteomics
Volume	11
Issue number	1
DOIs	https://doi.org/10.1186/1559-0275-11-17
Publication status	Published - Apr 2014

Keywords

2D-gel electrophoresis
Peritoneal dialysis effluent
Protein identification
Proteomics

Access to Document

10.1186/1559-0275-11-17

Cite this

@article{ea6e5ed3bd414ffd86a2bf4c95d1059a,

title = "Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response",

abstract = "BACKGROUND Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. RESULTS A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. CONCLUSIONS Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.",

keywords = "2D-gel electrophoresis, Peritoneal dialysis effluent, Protein identification, Proteomics",

author = "Elisabete Oliveira and Araujo, {Jose E.} and Silvana Gomez-Meire and Carlos Lodeiro and Cristina Perez-Melon and Elena Iglesias-Lamas and Alfonso Otero-Glez and Capelo, {Jos{\'e} Luis} and Santos, {Hugo M.}",

note = "SCOPUSID:84903776579 PMID:24742231 WOS:000209649600009",

year = "2014",

month = apr,

doi = "10.1186/1559-0275-11-17",

language = "English",

volume = "11",

pages = "17",

journal = "Clinical Proteomics",

issn = "1542-6416",

publisher = "BioMed Central (BMC)",

number = "1",

}

TY - JOUR

T1 - Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response

AU - Oliveira, Elisabete

AU - Araujo, Jose E.

AU - Gomez-Meire, Silvana

AU - Lodeiro, Carlos

AU - Perez-Melon, Cristina

AU - Iglesias-Lamas, Elena

AU - Otero-Glez, Alfonso

AU - Capelo, José Luis

AU - Santos, Hugo M.

N1 - SCOPUSID:84903776579 PMID:24742231 WOS:000209649600009

PY - 2014/4

Y1 - 2014/4

N2 - BACKGROUND Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. RESULTS A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. CONCLUSIONS Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.

AB - BACKGROUND Peritoneal dialysis (PD) is a form of renal replacement used for advanced chronic kidney disease. PD effluent holds a great potential for biomarker discovery for diagnosis and prognosis. In this study a novel approach to unravelling the proteome of PD effluent based-on dithiothreitol depletion followed by 2D-SDS-PAGE and protein identification using tandem mass spectrometry is proposed. RESULTS A total of 49 spots were analysed revealing 25 proteins differentially expressed, among them many proteins involved in calcium regulation. CONCLUSIONS Remarkably, a group of proteins dealing with calcium metabolism and calcium regulation has been found to be lost through peritoneal dialysate effluent, giving thus a potential explanation to the calcification of soft tissues in patients subjected to peritoneal dialysis and kidney injury. Comparison of literature dealing with PD is difficult due to differences in sample treatment and analytical methodologies.

KW - 2D-gel electrophoresis

KW - Peritoneal dialysis effluent

KW - Protein identification

KW - Proteomics

U2 - 10.1186/1559-0275-11-17

DO - 10.1186/1559-0275-11-17

M3 - Article

C2 - 24742231

SN - 1542-6416

VL - 11

SP - 17

JO - Clinical Proteomics

JF - Clinical Proteomics

IS - 1

ER -

Proteomics analysis of the peritoneal dialysate effluent reveals the presence of calcium-regulation proteins and acute inflammatory response

Abstract

Keywords

Access to Document

Fingerprint

Cite this