Proteomic evaluation of wound-healing processes in potato (Solanum tuberosum L.) tuber tissue

Ines Maria Chaves, Carla Maria Pinheiro, Jorge Almiro Barcelo Caldeira Pinto Paiva, Sebastien Planchon, Kjell Sergeant, José Graça, Gonçalo Costa, Ana Maria Coelho, C. P. Ricardo

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Abstract

Proteins from potato (Solanum tuberosum L.) tuber slices, related to the wound-healing process, were separated by 2-DE and identified by an MS analysis in MS and MS/MS mode. Slicing triggered differentiation processes that lead to changes in metabolism, activation of defence and cell-wall reinforcement. Proteins related to storage, cell growth and division, cell structure, signal transduction, energy production, disease/defence mechanisms and secondary metabolism were detected. Image analysis of the 2-DE gels revealed a time-dependent change in the complexity of the polypeptide patterns. By microscopic observation the polyalyphatic domain of suberin was dearly visible by D4, indicating that a dosing layer (primary suberisation) was formed by then. A PCA of the six sampling dates revealed two time phases, D0-D2 and D4-D8, with a border position between D2 and D4. Moreover, a PCA of differentially expressed proteins indicated the existence of a succession of proteomic events leading to wound-periderm reconstruction. Some late-expressed proteins (D6-D8), including a suberisation-associated anionic peroxidase, have also been identified in the native periderm. Despite this, protein patterns of D8 slices and native periderm were still different, suggesting that the processes of wound-periderm formation are extended in time and not fully equivalent. The information presented in this study gives dues for further work on wound healing-periderm formation processes.
Original languageUnknown
Pages (from-to)4154-4175
JournalProteomics
Volume9
Issue number17
DOIs
Publication statusPublished - 1 Jan 2009

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