Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Rickettsiales Ehrlichia ruminantium (ER) is the causative agent of heartwater, fatal tick-borne borne disease of livestock in sub-Saharan Africa and in the Caribbean, posing strong economical constraints to livestock production. In an attempt to identify the most prominent proteins expressed by this bacterium, especially those encoded by the major antigenic protein 1 (map1)multigene family, a proteome map of ER cultivated in endothelial cells was constructed by using two dimensional gel electrophoresis combined with mass spectrometry. Among the sixty-four spots detected, we could identify only four proteins from the MAP1-family; the other proteins detected were mainly related to energy, amino acid and general metabolism (26%), to protein turnover, chaperones and survival (21%) and to information processes (14%) or classified as hypothetical proteins (23%). Additional studies on MAP1-family protein using immunochemical labeling also revealed that these proteins are differentially expressed along the bacterium life cycle, presenting different structural organization. Interestingly, when infectious elementary bodies (EBs) are released from host cells, MAP1 appears to be organized in SDS and heat- resistant dimers and trimers stabilized by disulfide bridges. Overall, the results presented herein not only reveal the first partial proteome map of ER but provide new insights on the expression ER MAP1-family proteins in host endothelial cells
Original languageUnknown
Pages (from-to)305-314
JournalVeterinary Microbiology
Volume156
Issue number3-4
DOIs
Publication statusPublished - 1 Jan 2012

Cite this

@article{2aff445b733d4da795abf26d8cb5a09c,
title = "Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins",
abstract = "The Rickettsiales Ehrlichia ruminantium (ER) is the causative agent of heartwater, fatal tick-borne borne disease of livestock in sub-Saharan Africa and in the Caribbean, posing strong economical constraints to livestock production. In an attempt to identify the most prominent proteins expressed by this bacterium, especially those encoded by the major antigenic protein 1 (map1)multigene family, a proteome map of ER cultivated in endothelial cells was constructed by using two dimensional gel electrophoresis combined with mass spectrometry. Among the sixty-four spots detected, we could identify only four proteins from the MAP1-family; the other proteins detected were mainly related to energy, amino acid and general metabolism (26{\%}), to protein turnover, chaperones and survival (21{\%}) and to information processes (14{\%}) or classified as hypothetical proteins (23{\%}). Additional studies on MAP1-family protein using immunochemical labeling also revealed that these proteins are differentially expressed along the bacterium life cycle, presenting different structural organization. Interestingly, when infectious elementary bodies (EBs) are released from host cells, MAP1 appears to be organized in SDS and heat- resistant dimers and trimers stabilized by disulfide bridges. Overall, the results presented herein not only reveal the first partial proteome map of ER but provide new insights on the expression ER MAP1-family proteins in host endothelial cells",
keywords = "Two-dimensional electrophoresis, Proteome, Mass spectrometry, MAP1-family proteins, Ehrlichia ruminantium",
author = "Carrondo, {Manuel Jos{\'e} Teixeira} and Montes, {Ana Catarina} and Coelho, {Ana Maria} and Alves, {Paula Maria}",
year = "2012",
month = "1",
day = "1",
doi = "10.1016/j.vetmic.2011.11.022",
language = "Unknown",
volume = "156",
pages = "305--314",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier Science B.V., Amsterdam.",
number = "3-4",

}

TY - JOUR

T1 - Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins

AU - Carrondo, Manuel José Teixeira

AU - Montes, Ana Catarina

AU - Coelho, Ana Maria

AU - Alves, Paula Maria

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The Rickettsiales Ehrlichia ruminantium (ER) is the causative agent of heartwater, fatal tick-borne borne disease of livestock in sub-Saharan Africa and in the Caribbean, posing strong economical constraints to livestock production. In an attempt to identify the most prominent proteins expressed by this bacterium, especially those encoded by the major antigenic protein 1 (map1)multigene family, a proteome map of ER cultivated in endothelial cells was constructed by using two dimensional gel electrophoresis combined with mass spectrometry. Among the sixty-four spots detected, we could identify only four proteins from the MAP1-family; the other proteins detected were mainly related to energy, amino acid and general metabolism (26%), to protein turnover, chaperones and survival (21%) and to information processes (14%) or classified as hypothetical proteins (23%). Additional studies on MAP1-family protein using immunochemical labeling also revealed that these proteins are differentially expressed along the bacterium life cycle, presenting different structural organization. Interestingly, when infectious elementary bodies (EBs) are released from host cells, MAP1 appears to be organized in SDS and heat- resistant dimers and trimers stabilized by disulfide bridges. Overall, the results presented herein not only reveal the first partial proteome map of ER but provide new insights on the expression ER MAP1-family proteins in host endothelial cells

AB - The Rickettsiales Ehrlichia ruminantium (ER) is the causative agent of heartwater, fatal tick-borne borne disease of livestock in sub-Saharan Africa and in the Caribbean, posing strong economical constraints to livestock production. In an attempt to identify the most prominent proteins expressed by this bacterium, especially those encoded by the major antigenic protein 1 (map1)multigene family, a proteome map of ER cultivated in endothelial cells was constructed by using two dimensional gel electrophoresis combined with mass spectrometry. Among the sixty-four spots detected, we could identify only four proteins from the MAP1-family; the other proteins detected were mainly related to energy, amino acid and general metabolism (26%), to protein turnover, chaperones and survival (21%) and to information processes (14%) or classified as hypothetical proteins (23%). Additional studies on MAP1-family protein using immunochemical labeling also revealed that these proteins are differentially expressed along the bacterium life cycle, presenting different structural organization. Interestingly, when infectious elementary bodies (EBs) are released from host cells, MAP1 appears to be organized in SDS and heat- resistant dimers and trimers stabilized by disulfide bridges. Overall, the results presented herein not only reveal the first partial proteome map of ER but provide new insights on the expression ER MAP1-family proteins in host endothelial cells

KW - Two-dimensional electrophoresis

KW - Proteome

KW - Mass spectrometry

KW - MAP1-family proteins

KW - Ehrlichia ruminantium

U2 - 10.1016/j.vetmic.2011.11.022

DO - 10.1016/j.vetmic.2011.11.022

M3 - Article

VL - 156

SP - 305

EP - 314

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -