Protein-responsive polymers for point-of care detection of cardiac biomarker

Felismina T. C. Moreira, Sanjiv Sharma, Rosa A. F. Dutra, João Paulo da Costa de Noronha, Anthony E. G. Cass, M. Goreti F Sales

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon- sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications. Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate- rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The devices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 g/mL, respectively, with detection limits of 1.5 and 0.8 g/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivity and selectivity.
Original languageEnglish
Pages (from-to)123-132
JournalSensors And Actuators B-Chemical
Volume196
DOIs
Publication statusPublished - Jun 2014

Keywords

  • Biomimetic antibodies
  • Screen-printed electrodes
  • Biosensor
  • Electropolymerization
  • Molecular imprinting
  • Myoglobin

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