TY - JOUR
T1 - Protein-responsive polymers for point-of care detection of cardiac biomarker
AU - Moreira, Felismina T. C.
AU - Sharma, Sanjiv
AU - Dutra, Rosa A. F.
AU - Noronha, João Paulo da Costa de
AU - Cass, Anthony E. G.
AU - Sales, M. Goreti F
PY - 2014/6
Y1 - 2014/6
N2 - This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon- sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications. Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate- rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The devices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 g/mL, respectively, with detection limits of 1.5 and 0.8 g/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivity and selectivity.
AB - This work describes a novel use for the polymeric film, poly(o-aminophenol) (PAP) that was made respon- sive to a specific protein. This was achieved through templated electropolymerization of aminophenol (AP) in the presence of protein. The procedure involved adsorbing protein on the electrode surface and thereafter electroploymerizing the aminophenol. Proteins embedded at the outer surface of the polymeric film were digested by proteinase K and then washed away thereby creating vacant sites. The capacity of the template film to specifically rebind protein was tested with myoglobin (Myo), a cardiac biomarker for ischemia. The films acted as biomimetic artificial antibodies and were produced on a gold (Au) screen printed electrode (SPE), as a step towards disposable sensors to enable point-of-care applications. Raman spectroscopy was used to follow the surface modification of the Au-SPE. The ability of the mate- rial to rebind Myo was measured by electrochemical techniques, namely electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The devices displayed linear responses to Myo in EIS and SWV assays down to 4.0 and 3.5 g/mL, respectively, with detection limits of 1.5 and 0.8 g/mL. Good selectivity was observed in the presence of troponin T (TnT) and creatine kinase (CKMB) in SWV assays, and accurate results were obtained in applications to spiked serum. The sensor described in this work is a potential tool for screening Myo in point-of-care due to the simplicity of fabrication, disposability, short time response, low cost, good sensitivity and selectivity.
KW - Biomimetic antibodies
KW - Screen-printed electrodes
KW - Biosensor
KW - Electropolymerization
KW - Molecular imprinting
KW - Myoglobin
U2 - 10.1016/j.snb.2014.01.038
DO - 10.1016/j.snb.2014.01.038
M3 - Article
SN - 0925-4005
VL - 196
SP - 123
EP - 132
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -