Protein changes during programmed cell death in tobacco

Ines Maria Chaves, Marta Alves, Dina Carrilho, Maria C. Duque- Magalhães, C. P. Ricardo, Ana Paula Regalado

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Abstract

Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.
Original languageUnknown
Pages (from-to)153-158
JournalBiologia Plantarum
Volume55
Issue number1
DOIs
Publication statusPublished - 1 Jan 2011

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