Protein changes during programmed cell death in tobacco

Ines Maria Chaves; Marta Alves; Dina Carrilho; Maria C. Duque- Magalhães; C. P. Ricardo; Ana Paula Regalado

doi:10.1007/s10535-011-0021-y

Protein changes during programmed cell death in tobacco

Ines Maria Chaves, Marta Alves, Dina Carrilho, Maria C. Duque- Magalhães, C. P. Ricardo, Ana Paula Regalado

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.

Original language	Unknown
Pages (from-to)	153-158
Journal	Biologia Plantarum
Volume	55
Issue number	1
DOIs	https://doi.org/10.1007/s10535-011-0021-y
Publication status	Published - 1 Jan 2011

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10.1007/s10535-011-0021-y

Cite this

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title = "Protein changes during programmed cell death in tobacco",

abstract = "Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.",

keywords = "HEAT-SHOCK, YARIV REAGENT, ARABIDOPSIS-THALIANA, PLANT-CELLS, GENE-EXPRESSION, HYDROGEN-PEROXIDE, DEPENDENT MANNER, SUSPENSION-CULTURED CELLS, TRACHEARY ELEMENT DIFFERENTIATION, HYPERSENSITIVE RESPONSE",

author = "Chaves, {Ines Maria} and Marta Alves and Dina Carrilho and {Duque- Magalh{\~a}es}, {Maria C.} and Ricardo, {C. P.} and Regalado, {Ana Paula}",

year = "2011",

month = jan,

day = "1",

doi = "10.1007/s10535-011-0021-y",

language = "Unknown",

volume = "55",

pages = "153--158",

journal = "Biologia Plantarum",

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TY - JOUR

T1 - Protein changes during programmed cell death in tobacco

AU - Chaves, Ines Maria

AU - Alves, Marta

AU - Carrilho, Dina

AU - Duque- Magalhães, Maria C.

AU - Ricardo, C. P.

AU - Regalado, Ana Paula

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.

AB - Programmed cell death (PCD) was induced by the Yariv reagent in Nicotiana tabacum cv. Bright Yellow-2 cell suspension. The analyses of proteins extracts by 2-D electrophoresis clearly show massive protein degradation which was mainly due to cysteine protease activity. In contrast, some proteins remained unchanged up to 72 h after PCD induction. Peptide mass fingerprints of these proteins, obtained by MALDI-TOF, identified calreticulin, heat shock protein (HSP) 60, HSP70, malate dehydrogenase and mitochondrial ATP synthase beta-subunit.

KW - HEAT-SHOCK

KW - YARIV REAGENT

KW - ARABIDOPSIS-THALIANA

KW - PLANT-CELLS

KW - GENE-EXPRESSION

KW - HYDROGEN-PEROXIDE

KW - DEPENDENT MANNER

KW - SUSPENSION-CULTURED CELLS

KW - TRACHEARY ELEMENT DIFFERENTIATION

KW - HYPERSENSITIVE RESPONSE

U2 - 10.1007/s10535-011-0021-y

DO - 10.1007/s10535-011-0021-y

M3 - Article

SN - 0006-3134

VL - 55

SP - 153

EP - 158

JO - Biologia Plantarum

JF - Biologia Plantarum

IS - 1

ER -