Protective role of ortho-substituted Mn(III) N-alkylpyridylporphyrins against the oxidative injury induced by tert-butylhydroperoxide

Ana S. Fernandes, Jorge Gaspar, M. Fátima Cabral, José Rueff, Matilde Castro, Ines Batinic-Haberle, Judite Costa, Nuno G. Oliveira

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The present work addresses the role of two ortho-substituted Mn(III) N-alkylpyridylporphyrins, alkyl being ethyl in MnTE-2-PyP5 and n-hexyl in MnTnHex-2-PyP5, on the protection against the oxidant tert-butylhydroperoxide (TBHP). Their protective role was studied in V79 cells using endpoints of cell viability (MTT and crystal violet assays), intracellular O2• generation (dihydroethidium assay) and glutathione status (DTNB and monochlorobimane assays). MnPs per se did not show cytotoxicity (up to 25 μM, 24 h). The exposure to TBHP resulted in a significant decrease in cell viability and in an increase in the intracellular O2 levels. Also, TBHP depleted total and reduced glutathione and increased GSSG. The two MnPs counteracted remarkably the effects of TBHP. Even at low concentrations, both MnPs were protective in terms of cell viability and abrogated the intracellular O2 increase in a significant way. Also, they augmented markedly the total and reduced glutathione contents in TBHP-treated cells, highlighting the multiple mechanisms of protection of these SOD mimics, which at least in part may be ascribed to their electron-donating ability.

Original languageEnglish
Pages (from-to)430-440
Number of pages11
JournalFree Radical Research
Volume44
Issue number4
DOIs
Publication statusPublished - 19 Mar 2010

Fingerprint

tert-Butylhydroperoxide
Cells
Wounds and Injuries
Glutathione
Assays
Cell Survival
Dithionitrobenzoic Acid
Gentian Violet
Glutathione Disulfide
Cytotoxicity
Oxidants
Electrons

Keywords

  • Cytotoxicity
  • Glutathione
  • MnTE-2-PyP
  • MnTnHex-2-PyP
  • Superoxide dismutase mimetic
  • Tert- butylhydroperoxide

Cite this

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Protective role of ortho-substituted Mn(III) N-alkylpyridylporphyrins against the oxidative injury induced by tert-butylhydroperoxide. / Fernandes, Ana S.; Gaspar, Jorge; Cabral, M. Fátima; Rueff, José; Castro, Matilde; Batinic-Haberle, Ines; Costa, Judite; Oliveira, Nuno G.

In: Free Radical Research, Vol. 44, No. 4, 19.03.2010, p. 430-440.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective role of ortho-substituted Mn(III) N-alkylpyridylporphyrins against the oxidative injury induced by tert-butylhydroperoxide

AU - Fernandes, Ana S.

AU - Gaspar, Jorge

AU - Cabral, M. Fátima

AU - Rueff, José

AU - Castro, Matilde

AU - Batinic-Haberle, Ines

AU - Costa, Judite

AU - Oliveira, Nuno G.

PY - 2010/3/19

Y1 - 2010/3/19

N2 - The present work addresses the role of two ortho-substituted Mn(III) N-alkylpyridylporphyrins, alkyl being ethyl in MnTE-2-PyP5 and n-hexyl in MnTnHex-2-PyP5, on the protection against the oxidant tert-butylhydroperoxide (TBHP). Their protective role was studied in V79 cells using endpoints of cell viability (MTT and crystal violet assays), intracellular O2• generation (dihydroethidium assay) and glutathione status (DTNB and monochlorobimane assays). MnPs per se did not show cytotoxicity (up to 25 μM, 24 h). The exposure to TBHP resulted in a significant decrease in cell viability and in an increase in the intracellular O2• levels. Also, TBHP depleted total and reduced glutathione and increased GSSG. The two MnPs counteracted remarkably the effects of TBHP. Even at low concentrations, both MnPs were protective in terms of cell viability and abrogated the intracellular O2• increase in a significant way. Also, they augmented markedly the total and reduced glutathione contents in TBHP-treated cells, highlighting the multiple mechanisms of protection of these SOD mimics, which at least in part may be ascribed to their electron-donating ability.

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