Protective role of ortho-substituted Mn(III) N-alkylpyridylporphyrins against the oxidative injury induced by tert-butylhydroperoxide

Ana S. Fernandes, Jorge Gaspar, M. Fátima Cabral, José Rueff, Matilde Castro, Ines Batinic-Haberle, Judite Costa, Nuno G. Oliveira

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23 Citations (Scopus)

Abstract

The present work addresses the role of two ortho-substituted Mn(III) N-alkylpyridylporphyrins, alkyl being ethyl in MnTE-2-PyP5 and n-hexyl in MnTnHex-2-PyP5, on the protection against the oxidant tert-butylhydroperoxide (TBHP). Their protective role was studied in V79 cells using endpoints of cell viability (MTT and crystal violet assays), intracellular O2• generation (dihydroethidium assay) and glutathione status (DTNB and monochlorobimane assays). MnPs per se did not show cytotoxicity (up to 25 μM, 24 h). The exposure to TBHP resulted in a significant decrease in cell viability and in an increase in the intracellular O2 levels. Also, TBHP depleted total and reduced glutathione and increased GSSG. The two MnPs counteracted remarkably the effects of TBHP. Even at low concentrations, both MnPs were protective in terms of cell viability and abrogated the intracellular O2 increase in a significant way. Also, they augmented markedly the total and reduced glutathione contents in TBHP-treated cells, highlighting the multiple mechanisms of protection of these SOD mimics, which at least in part may be ascribed to their electron-donating ability.

Original languageEnglish
Pages (from-to)430-440
Number of pages11
JournalFree Radical Research
Volume44
Issue number4
DOIs
Publication statusPublished - 19 Mar 2010

Keywords

  • Cytotoxicity
  • Glutathione
  • MnTE-2-PyP
  • MnTnHex-2-PyP
  • Superoxide dismutase mimetic
  • Tert- butylhydroperoxide

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