TY - JOUR
T1 - Properties of novel international drug-resistant pneumococcal clones identified in day-care centers of Lisbon, Portugal
AU - Sousa, Natacha G.
AU - Sá-Leão, Raquel
AU - Crisóstomo, M. Inês
AU - Simas, Carla
AU - Nunes, Sónia
AU - Frazão, Nelson
AU - Carriço, João A.
AU - Mato, Rosario
AU - Santos-Sanches, Ilda
AU - De Lencastre, Hermínia
PY - 2005/9
Y1 - 2005/9
N2 - In this study, 61 drug-resistant Streptococcus pneumoniae strains were characterized by multilocus sequence typing (MLST). These strains were representatives of 26 major clones (defined using pulsed-field gel electrophoresis) accounting for 93% of the 1,285 drug-resistant Streptococcus pneumoniae isolates recovered from the nasopharynges of healthy children attending day-care centers in Lisbon during 2001 to 2003. Using MLST, 13 of the 26 clones were found to be identical or closely related to 11 Pneumococcal Molecular Epidemiology Network (PMEN) clones, 4 clones were found to be unique as there were no identical or highly related allelic profiles deposited in the MLST database, and the remaining 9 clones had sequence types that matched or differed at a single or double locus from allelic profiles available in the MLST database. These nine clones were of serotypes 33F, 10A, 19A, 19F, 6A, 20, 24F, and 3, one was nontypeable, and, by MLST, they were found to be identical or highly related to isolates from disease origin that were dispersed internationally. Since the majority of these clones had serotypes that are not included in the 7-valent conjugate pneumococcal vaccine, monitoring of these clones is important for surveying their possible spread in the future. We propose the inclusion of these novel international clones in the PMEN.
AB - In this study, 61 drug-resistant Streptococcus pneumoniae strains were characterized by multilocus sequence typing (MLST). These strains were representatives of 26 major clones (defined using pulsed-field gel electrophoresis) accounting for 93% of the 1,285 drug-resistant Streptococcus pneumoniae isolates recovered from the nasopharynges of healthy children attending day-care centers in Lisbon during 2001 to 2003. Using MLST, 13 of the 26 clones were found to be identical or closely related to 11 Pneumococcal Molecular Epidemiology Network (PMEN) clones, 4 clones were found to be unique as there were no identical or highly related allelic profiles deposited in the MLST database, and the remaining 9 clones had sequence types that matched or differed at a single or double locus from allelic profiles available in the MLST database. These nine clones were of serotypes 33F, 10A, 19A, 19F, 6A, 20, 24F, and 3, one was nontypeable, and, by MLST, they were found to be identical or highly related to isolates from disease origin that were dispersed internationally. Since the majority of these clones had serotypes that are not included in the 7-valent conjugate pneumococcal vaccine, monitoring of these clones is important for surveying their possible spread in the future. We propose the inclusion of these novel international clones in the PMEN.
UR - http://www.scopus.com/inward/record.url?scp=24744456343&partnerID=8YFLogxK
U2 - 10.1128/JCM.43.9.4696-4703.2005
DO - 10.1128/JCM.43.9.4696-4703.2005
M3 - Article
C2 - 16145129
SN - 0095-1137
VL - 43
SP - 4696
EP - 4703
JO - Journal Of Clinical Microbiology
JF - Journal Of Clinical Microbiology
IS - 9
ER -