Prognostic value of microRNA-203a expression in breast cancer

Bruno Costa Gomes, Manuela Martins, Paulina Lopes, Inês Morujão, M Oliveira, António Araújo, J. Rueff, AS Rodrigues

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Tumor heterogeneity and the poor outcome of breast cancer (BC) patients have led researchers to define new markers of this disease. In recent years, microRNA expression patterns have proven to be valuable disease indicators. The level of miR-203a, in particular, was shown to be altered in different types of cancer. The objective of the present study was to assess the relationship between miR-203a expression and clinicopathological features of BC in a Portuguese cohort. The expression levels of miR-203a were analyzed in 109 formalin-fixed paraffin-embedded paired normal and tumor tissue samples. Significant overexpression of miR-203a in the tumor tissues was found (1.7-fold higher) compared to the expression in the normal adjacent tissues (p=0.003). In addition, several clinicopathological characteristics presented an association with higher miR-203a expression levels. Tumors with diameter ≤18.5 mm (1.5-fold; p=0.019), tumors positive for estrogen receptor (fold-change, 1.71; p=0.042), progesterone receptor (fold-change, 1.50; p=0.046) and negative for HER2 (fold-change, 1.50; p=0.016) and high Ki-67 index (fold-change, 2.60; p=0.024) presented a significant difference in miR-203a expression compared with adjacent normal tissues. Tumors without invasion of lymph nodes also presented higher expression of miR-203a (fold-change, 2.40; p=0.004). With regard to histological classification, ductal carcinomas in situ (fold-change, 2.20; p=0.028) and invasive carcinoma NOS (fold-change, 1.71; p = 0.009) displayed significantly higher expression of miR-203a. Moreover, we found a significant downregulation of miR-203a with increased stage in invasive lobular carcinomas, suggesting that miR-203a could represent a potential marker to discriminate stages in invasive lobular carcinomas.

Original languageEnglish
Pages (from-to)1748-1756
Number of pages9
JournalOncology Reports
Volume36
Issue number3
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

MicroRNAs
Breast Neoplasms
Neoplasms
Lobular Carcinoma
Carcinoma, Intraductal, Noninfiltrating
Progesterone Receptors
Estrogen Receptors
Paraffin
Formaldehyde
Down-Regulation
Lymph Nodes
Research Personnel
Carcinoma

Keywords

  • Breast cancer
  • Estrogen receptor
  • HER2
  • mIR-203a
  • Progesterone receptor
  • RT-qPCR

Cite this

Gomes, Bruno Costa ; Martins, Manuela ; Lopes, Paulina ; Morujão, Inês ; Oliveira, M ; Araújo, António ; Rueff, J. ; Rodrigues, AS. / Prognostic value of microRNA-203a expression in breast cancer. In: Oncology Reports. 2016 ; Vol. 36, No. 3. pp. 1748-1756.
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Prognostic value of microRNA-203a expression in breast cancer. / Gomes, Bruno Costa; Martins, Manuela; Lopes, Paulina; Morujão, Inês; Oliveira, M; Araújo, António; Rueff, J.; Rodrigues, AS.

In: Oncology Reports, Vol. 36, No. 3, 01.09.2016, p. 1748-1756.

Research output: Contribution to journalArticle

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AU - Gomes, Bruno Costa

AU - Martins, Manuela

AU - Lopes, Paulina

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AB - Tumor heterogeneity and the poor outcome of breast cancer (BC) patients have led researchers to define new markers of this disease. In recent years, microRNA expression patterns have proven to be valuable disease indicators. The level of miR-203a, in particular, was shown to be altered in different types of cancer. The objective of the present study was to assess the relationship between miR-203a expression and clinicopathological features of BC in a Portuguese cohort. The expression levels of miR-203a were analyzed in 109 formalin-fixed paraffin-embedded paired normal and tumor tissue samples. Significant overexpression of miR-203a in the tumor tissues was found (1.7-fold higher) compared to the expression in the normal adjacent tissues (p=0.003). In addition, several clinicopathological characteristics presented an association with higher miR-203a expression levels. Tumors with diameter ≤18.5 mm (1.5-fold; p=0.019), tumors positive for estrogen receptor (fold-change, 1.71; p=0.042), progesterone receptor (fold-change, 1.50; p=0.046) and negative for HER2 (fold-change, 1.50; p=0.016) and high Ki-67 index (fold-change, 2.60; p=0.024) presented a significant difference in miR-203a expression compared with adjacent normal tissues. Tumors without invasion of lymph nodes also presented higher expression of miR-203a (fold-change, 2.40; p=0.004). With regard to histological classification, ductal carcinomas in situ (fold-change, 2.20; p=0.028) and invasive carcinoma NOS (fold-change, 1.71; p = 0.009) displayed significantly higher expression of miR-203a. Moreover, we found a significant downregulation of miR-203a with increased stage in invasive lobular carcinomas, suggesting that miR-203a could represent a potential marker to discriminate stages in invasive lobular carcinomas.

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