TY - JOUR
T1 - Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology
AU - Cruz, Madalena V.
AU - Paiva, Alexandre
AU - Lisboa, Pedro
AU - Freitas, Filomena
AU - Alves, Vitor D.
AU - Simões, Pedro Miguel Calado
AU - Barreiros, Susana Filipe
AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda
N1 - Sem PDF.
This work was developed as a Quadro de Referencia Estrategica Nacional (QREN) project in collaboration with the Portuguese coffee company - NovaDelta, Comercio e Industria de Cafes, S.A. (Campo Maior, Portugal). Filomena Freitas, Alexandre Paiva, Madalena V. Cruz and Pedro Lisboa acknowledge the financial support of Fundacao para a Ciencia e a Tecnologia (Portugal) through, respectively, Post-Doctoral fellowships SFRH/BPD/72280/2010 and SFRH/BPD/44946/2008, and PhD fellowships SFRH/BD/72142/2010 and SFRH/BDE/51890/2012, as well as the project PEst-C/EQB/LA0006/2013. The authors thank Prof. Ana Maria Ramos for the determination of the molecular weight of the polymer.
PY - 2014/4
Y1 - 2014/4
N2 - Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO2) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg(-1) CO2/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL(-1) with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL(-1)day(-1) and 0.77gg(-1), respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34×10(5) and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.
AB - Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO2) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg(-1) CO2/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL(-1) with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL(-1)day(-1) and 0.77gg(-1), respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34×10(5) and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.
KW - Cupriavidus necator
KW - Polyhydroxyalkanoates (PHAs)
KW - Spent coffee grounds (SCG)
KW - Supercritical fluid extraction (SFE)
KW - Triglycerides
U2 - 10.1016/j.biortech.2014.02.013
DO - 10.1016/j.biortech.2014.02.013
M3 - Article
C2 - 24594316
SN - 0960-8524
VL - 157
SP - 360
EP - 363
JO - Bioresource Technology
JF - Bioresource Technology
ER -