Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology

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Abstract

Spent coffee grounds (SCG) oil was obtained by supercritical carbon dioxide (scCO2) extraction in a pilot plant apparatus, with an oil extraction yield of 90% at a 35kgkg(-1) CO2/SCG ratio. Cupriavidus necator DSM 428 was cultivated in 2L bioreactor using extracted SCG oil as sole carbon source for production of polyhydroxyalkanoates. The culture reached a cell dry weight of 16.7gL(-1) with a polymer content of 78.4% (w/w). The volumetric polymer productivity and oil yield were 4.7gL(-1)day(-1) and 0.77gg(-1), respectively. The polymer produced was a homopolymer of 3-hydroxybutyrate with an average molecular weight of 2.34×10(5) and a polydispersity index of 1.2. The polymer exhibited brittle behaviour, with very low elongation at break (1.3%), tensile strength at break of 16MPa and Young's Modulus of 1.0GPa. Results show that SCG can be a bioresource for polyhydroxyalkanoates production with interesting properties.
Original languageEnglish
Pages (from-to)360-363
JournalBioresource Technology
Volume157
DOIs
Publication statusPublished - Apr 2014

Keywords

  • Cupriavidus necator
  • Polyhydroxyalkanoates (PHAs)
  • Spent coffee grounds (SCG)
  • Supercritical fluid extraction (SFE)
  • Triglycerides

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