TY - JOUR
T1 - Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas
T2 - Cultivation on fruit pulp waste and polymer characterizationchlororaphis subsp. aurantiaca
AU - Pereira, João R.
AU - Araújo, Diana
AU - Freitas, Patrícia
AU - Marques, Ana C.
AU - Alves, Vítor D.
AU - Sevrin, Chantal
AU - Grandfils, Christian
AU - Fortunato, Elvira
AU - Reis, Maria A. M.
AU - Freitas, Filomena
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50025%2F2020/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F147518%2F2019/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F140829%2F2018/PT#
info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F115173%2F2016/PT#
This project was supported by Unidade de Ciencias Biomoleculares Aplicadas (UCIBIO), Centro de Investigacao de Materiais vertical bar i3N and LEAF-Linking Landscape, Environment, Agriculture and Food, which are financed by national funds from FCT/MCTES (UID/04378/2020 and UID/AGR/04129/2020, respectively). Joao R. Pereira, Diana Araujo and Ana C. Marques acknowledge , respectively.
PY - 2021/1/15
Y1 - 2021/1/15
N2 - Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructose-rich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7 ± 0.1 mol% 3-hydroxydecanoate, 17.9 ± 1.0 mol% 3-hydroxyoctanoate, 14.5 ± 1.1 mol% 3-hydroxybutyrate, 11.1 ± 0.6 mol% 3-hydroxytetradecanoate, 10.1 ± 0.5 mol% 3-hydroxydodecanoate and 3.7 ± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0 ± 0.1 °C, respectively), and a degradation temperature of 300.0 ± 0.1 °C, coupled to a low crystallinity index (12.7 ± 2.7%), a molecular weight (Mw) of 1.34 × 105 ± 0.18 × 105 Da and a polydispersity index of 2.70 ± 0.03. The biopolymer's films were dense and had a smooth surface, as demonstrated by Scanning Electron Microscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging.
AB - Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructose-rich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7 ± 0.1 mol% 3-hydroxydecanoate, 17.9 ± 1.0 mol% 3-hydroxyoctanoate, 14.5 ± 1.1 mol% 3-hydroxybutyrate, 11.1 ± 0.6 mol% 3-hydroxytetradecanoate, 10.1 ± 0.5 mol% 3-hydroxydodecanoate and 3.7 ± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0 ± 0.1 °C, respectively), and a degradation temperature of 300.0 ± 0.1 °C, coupled to a low crystallinity index (12.7 ± 2.7%), a molecular weight (Mw) of 1.34 × 105 ± 0.18 × 105 Da and a polydispersity index of 2.70 ± 0.03. The biopolymer's films were dense and had a smooth surface, as demonstrated by Scanning Electron Microscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging.
KW - Fruit pulp waste
KW - Polyhydroxyalkanoates (PHA)
KW - Pseudomonas chlororaphis
UR - http://www.scopus.com/inward/record.url?scp=85097043214&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2020.11.162
DO - 10.1016/j.ijbiomac.2020.11.162
M3 - Article
C2 - 33249156
AN - SCOPUS:85097043214
SN - 0141-8130
VL - 167
SP - 85
EP - 92
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -