Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions

Nuno H C S Silva; Ricardo J B Pinto; Carmen S R Freire; Isabel M. Marrucho

doi:10.1016/j.colsurfb.2016.07.005

Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions

Nuno H C S Silva, Ricardo J B Pinto, Carmen S R Freire, Isabel M. Marrucho

Research output: Contribution to journal › Article

10 Citations (Scopus)

Abstract

Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2–3 h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5–1 μm in length and 0.02–0.1 μm in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology.

Original language	English
Pages (from-to)	36-44
Number of pages	9
Journal	Colloids And Surfaces B-Biointerfaces
Volume	147
DOIs	https://doi.org/10.1016/j.colsurfb.2016.07.005
Publication status	Published - 1 Nov 2016

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Silva, N. H. C. S., Pinto, R. J. B., Freire, C. S. R., & Marrucho, I. M. (2016). Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions. Colloids And Surfaces B-Biointerfaces, 147, 36-44. https://doi.org/10.1016/j.colsurfb.2016.07.005

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abstract = "Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2–3 h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5–1 μm in length and 0.02–0.1 μm in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology.",

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