Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source

Silvia Antunes, Filomena Freitas, Chantal Sevrin, Christian Grandfils, Maria A. M. Reis

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16 Citations (Scopus)


Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide. Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77�g�L−1) and overall volumetric productivity (2.92�g�L−1�d−1) were obtained with continuous substrate feeding at a constant flow rate of 11�g�h−1. The polymer produced had the typical FucoPol composition (37�mol% fucose, 27�mol% galactose, 23�mol% glucose and 12�mol% glucuronic acid, with an acyl groups content of 13�wt%). The average molecular weight was 4.4�נ106�Da and the polydispersity index was 1.2. This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.

Original languageEnglish
Pages (from-to)66-73
Number of pages8
JournalBioresource Technology
Publication statusPublished - 2017


  • Continuous feeding
  • DO-stat mode
  • Extracellular polysaccharide (EPS)
  • pH-stat mode
  • Tomato paste


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