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Pure glycerol and glycerol-rich product (GRP) obtained from the biodiesel industries were used as carbon source for the production of a new extracellular polysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682. The influence of temperature (20-40 degrees C) and pH (6.0-8.0) was studied. A temperature of 30 degrees C and pH control at 6.8 gave the maximum cell growth and EPS production. The culture attained a maximum cell dry weight (CDW) of 9.55 g l(-1) and an EPS concentration of 11.82 g l(-1) when cultivated with pure glycerol. GRP was a suitable carbon source, as shown by the slightly higher EPS concentration (12.18 g l(-1)). The EPS productivity obtained with GRP (3.85 g l(-1) d(-1)) was almost twice that obtained with pure glycerol (2.00 g l(-1) d(-1)). Also, the yield on glycerol was higher for the cultivation with GRP (0.36 g g(-1))than for pure glycerol (0.28 g g(-1)). The EPS was a high molecular weight heteropolysaccharide, composed by neutral sugars (37-80 wt% galactose, 2-30 wt% glucose, 0.5-25 wt% mannose and 0.5-20 wt% rhamnose) and containing acyl group substituents (pyruvil, acetyl and succinyl were identified). The EPS forms highly viscous aqueous dispersions with many potential commercial applications. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)297-305
JournalProcess Biochemistry
Issue number3
Publication statusPublished - 1 Jan 2010

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