25 Citations (Scopus)

Abstract

Pure glycerol and glycerol-rich product (GRP) obtained from the biodiesel industries were used as carbon source for the production of a new extracellular polysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682. The influence of temperature (20-40 degrees C) and pH (6.0-8.0) was studied. A temperature of 30 degrees C and pH control at 6.8 gave the maximum cell growth and EPS production. The culture attained a maximum cell dry weight (CDW) of 9.55 g l(-1) and an EPS concentration of 11.82 g l(-1) when cultivated with pure glycerol. GRP was a suitable carbon source, as shown by the slightly higher EPS concentration (12.18 g l(-1)). The EPS productivity obtained with GRP (3.85 g l(-1) d(-1)) was almost twice that obtained with pure glycerol (2.00 g l(-1) d(-1)). Also, the yield on glycerol was higher for the cultivation with GRP (0.36 g g(-1))than for pure glycerol (0.28 g g(-1)). The EPS was a high molecular weight heteropolysaccharide, composed by neutral sugars (37-80 wt% galactose, 2-30 wt% glucose, 0.5-25 wt% mannose and 0.5-20 wt% rhamnose) and containing acyl group substituents (pyruvil, acetyl and succinyl were identified). The EPS forms highly viscous aqueous dispersions with many potential commercial applications. (C) 2009 Elsevier Ltd. All rights reserved.
Original languageUnknown
Pages (from-to)297-305
JournalProcess Biochemistry
Volume45
Issue number3
DOIs
Publication statusPublished - 1 Jan 2010

Cite this

@article{38c2758454e842eaa7e34e1a6993f245,
title = "Production of a new exopolysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682 grown on glycerol",
abstract = "Pure glycerol and glycerol-rich product (GRP) obtained from the biodiesel industries were used as carbon source for the production of a new extracellular polysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682. The influence of temperature (20-40 degrees C) and pH (6.0-8.0) was studied. A temperature of 30 degrees C and pH control at 6.8 gave the maximum cell growth and EPS production. The culture attained a maximum cell dry weight (CDW) of 9.55 g l(-1) and an EPS concentration of 11.82 g l(-1) when cultivated with pure glycerol. GRP was a suitable carbon source, as shown by the slightly higher EPS concentration (12.18 g l(-1)). The EPS productivity obtained with GRP (3.85 g l(-1) d(-1)) was almost twice that obtained with pure glycerol (2.00 g l(-1) d(-1)). Also, the yield on glycerol was higher for the cultivation with GRP (0.36 g g(-1))than for pure glycerol (0.28 g g(-1)). The EPS was a high molecular weight heteropolysaccharide, composed by neutral sugars (37-80 wt{\%} galactose, 2-30 wt{\%} glucose, 0.5-25 wt{\%} mannose and 0.5-20 wt{\%} rhamnose) and containing acyl group substituents (pyruvil, acetyl and succinyl were identified). The EPS forms highly viscous aqueous dispersions with many potential commercial applications. (C) 2009 Elsevier Ltd. All rights reserved.",
keywords = "alginate, polysaccharide, azotobacter-vinelandii, production, molecular-weight, sphingomonas-paucimobilis, source, Glycerol-rich, Pseudomonas, gellan, crude, B-14682, carbon, NRRL, (EPS), product, tension, dissolved-oxygen, Extracellular, glycerol, xanthomonas-campestris, xanthan, Bioreactor, (GRP), oleovorans, gum",
author = "Filomena Freitas and Reis, {Maria D'ascens{\~a}o Carvalho Fernandes Miranda} and Oliveira, {Rui Manuel Freitas}",
year = "2010",
month = "1",
day = "1",
doi = "10.1016/j.procbio.2009.09.020",
language = "Unknown",
volume = "45",
pages = "297--305",
journal = "Process Biochemistry",
issn = "0032-9592",
publisher = "Elsevier Science B.V., Amsterdam.",
number = "3",

}

TY - JOUR

T1 - Production of a new exopolysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682 grown on glycerol

AU - Freitas, Filomena

AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda

AU - Oliveira, Rui Manuel Freitas

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Pure glycerol and glycerol-rich product (GRP) obtained from the biodiesel industries were used as carbon source for the production of a new extracellular polysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682. The influence of temperature (20-40 degrees C) and pH (6.0-8.0) was studied. A temperature of 30 degrees C and pH control at 6.8 gave the maximum cell growth and EPS production. The culture attained a maximum cell dry weight (CDW) of 9.55 g l(-1) and an EPS concentration of 11.82 g l(-1) when cultivated with pure glycerol. GRP was a suitable carbon source, as shown by the slightly higher EPS concentration (12.18 g l(-1)). The EPS productivity obtained with GRP (3.85 g l(-1) d(-1)) was almost twice that obtained with pure glycerol (2.00 g l(-1) d(-1)). Also, the yield on glycerol was higher for the cultivation with GRP (0.36 g g(-1))than for pure glycerol (0.28 g g(-1)). The EPS was a high molecular weight heteropolysaccharide, composed by neutral sugars (37-80 wt% galactose, 2-30 wt% glucose, 0.5-25 wt% mannose and 0.5-20 wt% rhamnose) and containing acyl group substituents (pyruvil, acetyl and succinyl were identified). The EPS forms highly viscous aqueous dispersions with many potential commercial applications. (C) 2009 Elsevier Ltd. All rights reserved.

AB - Pure glycerol and glycerol-rich product (GRP) obtained from the biodiesel industries were used as carbon source for the production of a new extracellular polysaccharide (EPS) by Pseudomonas oleovorans NRRL B-14682. The influence of temperature (20-40 degrees C) and pH (6.0-8.0) was studied. A temperature of 30 degrees C and pH control at 6.8 gave the maximum cell growth and EPS production. The culture attained a maximum cell dry weight (CDW) of 9.55 g l(-1) and an EPS concentration of 11.82 g l(-1) when cultivated with pure glycerol. GRP was a suitable carbon source, as shown by the slightly higher EPS concentration (12.18 g l(-1)). The EPS productivity obtained with GRP (3.85 g l(-1) d(-1)) was almost twice that obtained with pure glycerol (2.00 g l(-1) d(-1)). Also, the yield on glycerol was higher for the cultivation with GRP (0.36 g g(-1))than for pure glycerol (0.28 g g(-1)). The EPS was a high molecular weight heteropolysaccharide, composed by neutral sugars (37-80 wt% galactose, 2-30 wt% glucose, 0.5-25 wt% mannose and 0.5-20 wt% rhamnose) and containing acyl group substituents (pyruvil, acetyl and succinyl were identified). The EPS forms highly viscous aqueous dispersions with many potential commercial applications. (C) 2009 Elsevier Ltd. All rights reserved.

KW - alginate

KW - polysaccharide

KW - azotobacter-vinelandii

KW - production

KW - molecular-weight

KW - sphingomonas-paucimobilis

KW - source

KW - Glycerol-rich

KW - Pseudomonas

KW - gellan

KW - crude

KW - B-14682

KW - carbon

KW - NRRL

KW - (EPS)

KW - product

KW - tension

KW - dissolved-oxygen

KW - Extracellular

KW - glycerol

KW - xanthomonas-campestris

KW - xanthan

KW - Bioreactor

KW - (GRP)

KW - oleovorans

KW - gum

U2 - 10.1016/j.procbio.2009.09.020

DO - 10.1016/j.procbio.2009.09.020

M3 - Article

VL - 45

SP - 297

EP - 305

JO - Process Biochemistry

JF - Process Biochemistry

SN - 0032-9592

IS - 3

ER -