Production and crystallization of alpha-phosphoglucomutase from Lactococcus lactis

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Abstract

alpha-Phosphoglucomutase (alpha-PGM) is an enzyme that is essential for the growth of Lactococcus lactis. The enzyme links bacterial anabolism with sugar utilization through glycolysis by catalyzing the reversible interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate. The gene encoding alpha-PGM was cloned and overexpressed in L. lactis. The purified protein was functionally active and was crystallized with ammonium sulfate as a precipitant using vapour-diffusion and seeding techniques. Optimized crystals diffracted to 1.5 angstrom resolution at a synchrotron source.
Original languageUnknown
Pages (from-to)1113-1115
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume68
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2012

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