Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope

J M Marcelino; C Novo; J M Pereira; F Picotez; Adelaide Clemente; Nuno Taveira

doi:10.1089/088922201750461348

Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope

J M Marcelino, C Novo, J M Pereira, F Picotez, Adelaide Clemente, Nuno Taveira

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Abstract

One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

Original language	English
Pages (from-to)	1279-1283
Number of pages	5
Journal	AIDS Research and Human Retroviruses
Volume	Vol. 17
Issue number	n.º 13
DOIs	https://doi.org/10.1089/088922201750461348
Publication status	Published - 1 Sept 2001

Keywords

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antibody Specificity
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Epitopes
Gene Products, gag
HIV Antibodies
HIV Antigens
HIV-1
HIV-2
Humans
Mice
Mice, Inbred BALB C
Molecular Sequence Data
gag Gene Products, Human Immunodeficiency Virus
Journal Article

UN SDGs

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Marcelino, J. M., Novo, C., Pereira, J. M., Picotez, F., Clemente, A., & Taveira, N. (2001). Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope. AIDS Research and Human Retroviruses, Vol. 17(n.º 13), 1279-1283. https://doi.org/10.1089/088922201750461348

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title = "Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope",

abstract = "One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.",

keywords = "Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antibody Specificity, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Epitope Mapping, Epitopes, Gene Products, gag, HIV Antibodies, HIV Antigens, HIV-1, HIV-2, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, gag Gene Products, Human Immunodeficiency Virus, Journal Article",

author = "Marcelino, {J M} and C Novo and Pereira, {J M} and F Picotez and Adelaide Clemente and Nuno Taveira",

year = "2001",

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language = "English",

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Marcelino, JM , Novo, C , Pereira, JM, Picotez, F, Clemente, A & Taveira, N 2001, 'Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope', AIDS Research and Human Retroviruses, vol. Vol. 17, no. n.º 13, pp. 1279-1283. https://doi.org/10.1089/088922201750461348

Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope. / Marcelino, J M ; Novo, C ; Pereira, J M et al.
In: AIDS Research and Human Retroviruses, Vol. Vol. 17, No. n.º 13, 01.09.2001, p. 1279-1283.

Research output: Contribution to journal › Article › peer-review

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AU - Marcelino, J M

AU - Novo, C

AU - Pereira, J M

AU - Picotez, F

AU - Clemente, Adelaide

AU - Taveira, Nuno

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KW - Antibody Specificity

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KW - Epitope Mapping

KW - Epitopes

KW - Gene Products, gag

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SN - 0889-2229

VL - Vol. 17

SP - 1279

EP - 1283

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

IS - n.º 13

ER -

Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope

Abstract

Keywords

UN SDGs

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