Production and characterization of a mouse monoclonal antibody against the Gag p26 protein of human immunodeficiency virus type 2: identification of a new antigenic epitope

J M Marcelino, C Novo, J M Pereira, F Picotez, Adelaide Clemente, Nuno Taveira

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.

Original languageEnglish
Pages (from-to)1279-1283
Number of pages5
JournalAIDS Research and Human Retroviruses
VolumeVol. 17
Issue numbern.º 13
DOIs
Publication statusPublished - 1 Sep 2001

Keywords

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Epitopes
  • Gene Products, gag
  • HIV Antibodies
  • HIV Antigens
  • HIV-1
  • HIV-2
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • gag Gene Products, Human Immunodeficiency Virus
  • Journal Article

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