Processing of extracellular lipase of Lactobacillus plantarum: Involvement of a metalloprotease

Maria De Fátima Silva Lopes, Ana Lúcia Leitão, J. J. Figueiredo Marques, Manuel José Teixeira Carrondo, Maria Teresa Barreto Crespo

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9 Citations (Scopus)

Abstract

The hypothesis of a proteolytic involvement in the extracellular lipase processing of a strain of Lactobacillus plantarum was considered and tested. in vitro assays with acid proteases, cathepsin D and renin revealed that both did affect lipolytic activity positively. In vivo assays with growth in the presence of the protease inhibitors pepstatin, phenylmethylsulfonyl fluoride, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane and ethylenediaminetetraacetic acid showed that ethylenediaminetetraacetic acid did affect the pattern of the proteins that possess lipolytic activity. Therefore, it is suggested that a metalloprotease is involved in the processing of the extracellular lipases of L. plantarum, although other proteases can also be important. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)483-487
Number of pages5
JournalFEMS Microbiology Letters
Volume176
Issue number2
DOIs
Publication statusPublished - 15 Jul 1999

Keywords

  • Lactobacillus
  • Lipolytic activity
  • Metalloprotease

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