Different Mediterranean Basin limestones, like Calcario Anca (Portugal), Calcario Lioz (Portugal), Piedra San Cristobal (Spain), Piedra Escuzar (Spain) and Pietra di Lecce (Italy), have been widely used as building materials in the European architecture. The aim of this study was focused on biodeterioration, mainly on evaluation of the primary bioreceptivity of those materials. A set of samples was inoculated with a cultured photosynthetic biofilm under laboratory conditions. Several assessment tools were applied to monitor the colonization overtime of the different lithotypes. After 3 months of incubation the colonization occurred endolithically in some lithotypes, namely Piedra San Cristobal and Piedra Escuzar. Spectrophotometric determination of chlorophyll a was a useful analytical technique to achieve the total amount of photosynthetic biomass on rock substrates, demonstrating that Piedra Escuzar and Calcario Lioz were the highest and lowest bioreceptive lithotypes, respectively. Microscopic and image analyses were essential to understand the stone colonization process and its pattern of distribution. Physical stone parameters and exposure conditions were shown to play an important role in the establishment and development of photosynthetic colonization.
|Journal||LIMESTONE IN THE BUILT ENVIRONMENT: PRESENT DAY CHALLENGES FOR THE PRESERVATION OF THE PAST|
|Publication status||Published - 1 Jan 2010|