Preliminary structural characterization of human SOUL, a haem-binding protein

Filipe Freire; Maria João Romão; Anjos L. MacEdo; Susana S. Aveiro; Brian J. Goodfellow; Ana Luísa Carvalho

doi:10.1107/S174430910902291X

Preliminary structural characterization of human SOUL, a haem-binding protein

Filipe Freire, Maria João Romão, Anjos L. MacEdo, Susana S. Aveiro, Brian J. Goodfellow, Ana Luísa Carvalho

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Abstract

Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.

Original language	English
Pages (from-to)	723-726
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	65
Issue number	7
DOIs	https://doi.org/10.1107/S174430910902291X
Publication status	Published - 1 Jan 2009

Keywords

Haem-binding proteins
SOUL

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f-65-00723Final published version, 237 KBLicence: CC BY

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title = "Preliminary structural characterization of human SOUL, a haem-binding protein",

abstract = "Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 {\AA} and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 {\AA} resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.",

keywords = "Haem-binding proteins, SOUL",

author = "Filipe Freire and Rom{\~a}o, {Maria Jo{\~a}o} and MacEdo, {Anjos L.} and Aveiro, {Susana S.} and Goodfellow, {Brian J.} and Carvalho, {Ana Lu{\'i}sa}",

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AU - Freire, Filipe

AU - Romão, Maria João

AU - MacEdo, Anjos L.

AU - Aveiro, Susana S.

AU - Goodfellow, Brian J.

AU - Carvalho, Ana Luísa

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PY - 2009/1/1

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AB - Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.

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Preliminary structural characterization of human SOUL, a haem-binding protein

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