Preliminary structural characterization of human SOUL, a haem-binding protein

Filipe Freire, Maria João Romão, Anjos L. MacEdo, Susana S. Aveiro, Brian J. Goodfellow, Ana Luísa Carvalho

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Abstract

Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6422, with unit-cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models.

Original languageEnglish
Pages (from-to)723-726
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume65
Issue number7
DOIs
Publication statusPublished - 1 Jan 2009

Keywords

  • Haem-binding proteins
  • SOUL

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