Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Diogo Filipe; Ricardo Parreira; André Pereira; Nuno Galvão; José Manuel Cristóvão; Mónica Nunes; Maria Luísa Vieira; Lenea Campino; Carla Maia

doi:10.1016/j.vprsr.2021.100551

Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Diogo Filipe, Ricardo Parreira, André Pereira, Nuno Galvão, José Manuel Cristóvão, Mónica Nunes, Maria Luísa Vieira, Lenea Campino, Carla Maia

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.

Original language	English
Article number	100551
Pages (from-to)	100551
Journal	Veterinary Parasitology: Regional Studies and Reports
Volume	24
DOIs	https://doi.org/10.1016/j.vprsr.2021.100551
Publication status	Published - 1 Apr 2021

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10.1016/j.vprsr.2021.100551

https://linkinghub.elsevier.com/retrieve/pii/S2405939021000228

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Filipe, D., Parreira, R., Pereira, A., Galvão, N., Cristóvão, J. M., Nunes, M., Vieira, M. L., Campino, L., & Maia, C. (2021). Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal. Veterinary Parasitology: Regional Studies and Reports, 24, 100551. Article 100551. https://doi.org/10.1016/j.vprsr.2021.100551

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title = "Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal",

abstract = "The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.",

author = "Diogo Filipe and Ricardo Parreira and Andr{\'e} Pereira and Nuno Galv{\~a}o and Crist{\'o}v{\~a}o, {Jos{\'e} Manuel} and M{\'o}nica Nunes and Vieira, {Maria Lu{\'i}sa} and Lenea Campino and Carla Maia",

note = "Funding Information: Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia , I.P. (FCT) through contract GHTM-UID/Multi/04413/2013 . AP has the support of the Portuguese Ministry of Education and Science (via Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia, I.P.), through a PhD grant ( SFRH/BD/116516/2016 ). Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

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Filipe, D, Parreira, R, Pereira, A, Galvão, N, Cristóvão, JM, Nunes, M, Vieira, ML , Campino, L & Maia, C 2021, 'Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal', Veterinary Parasitology: Regional Studies and Reports, vol. 24, 100551, pp. 100551. https://doi.org/10.1016/j.vprsr.2021.100551

Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal. / Filipe, Diogo; Parreira, Ricardo; Pereira, André et al.
In: Veterinary Parasitology: Regional Studies and Reports, Vol. 24, 100551, 01.04.2021, p. 100551.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

AU - Filipe, Diogo

AU - Parreira, Ricardo

AU - Pereira, André

AU - Galvão, Nuno

AU - Cristóvão, José Manuel

AU - Nunes, Mónica

AU - Vieira, Maria Luísa

AU - Campino, Lenea

AU - Maia, Carla

N1 - Funding Information: Fundação para a Ciência e a Tecnologia , I.P. (FCT) through contract GHTM-UID/Multi/04413/2013 . AP has the support of the Portuguese Ministry of Education and Science (via Fundação para a Ciência e a Tecnologia, I.P.), through a PhD grant ( SFRH/BD/116516/2016 ). Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/4/1

Y1 - 2021/4/1

N2 - The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.

AB - The utility of mitochondrial cytochrome oxidase subunit I (COX1) and 16S ribosomal DNA (16S-rDNA) sequence analyses as a complementary/alternative tool to classical taxonomy, for the identification of some of the most prevalent hard tick species from Portugal was evaluated using BOLD-ID (COX1 only), BLASTn and phylogenetic tree reconstruction based on multiple nucleotide sequence alignments. Both molecular markers proved suitable for identifying ticks to a species level, but specific aspects that limit their resolving power must be considered. Their accuracy of tick identification in all life stages and of the other tick species described in the South of Europe is required.

U2 - 10.1016/j.vprsr.2021.100551

DO - 10.1016/j.vprsr.2021.100551

M3 - Article

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VL - 24

SP - 100551

JO - Veterinary Parasitology: Regional Studies and Reports

JF - Veterinary Parasitology: Regional Studies and Reports

M1 - 100551

ER -

Preliminary comparative analysis of the resolving power of COX1 and 16S-rDNA as molecular markers for the identification of ticks from Portugal

Abstract

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