Preferential sensitivity of acrocentric chromosomes to the aneugenic effect of colchicine

Helena Caria, Teresa Chaveca, José Rueff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In order to evaluate the predisposition to the aneuploidy-inducing agent colchicine (Col) on lymphocytes from trisomic 21 patients compared with their parents and with a control group of subjects without trisomic children, we performed the micronucleus (MN) assay associated with C-banding, CREST staining, and nucleolar organizing region (NOR)-banding. According to our results Col behaves as an aneugenic agent independently of the population studied for CREST and C-banding. The Col-induced MN exhibited a clear majority (>80%) of positive NOR-MN, meaning that they contain a NOR region transcriptionally active or inactive. The same data were observed in trisomic 21 individuals, their parents, and the control group, without significant differences between them. These results seem to suggest a preferential effect of the aneugen Col on acrocentric chromosomes in all of the three groups studied.

Original languageEnglish
Pages (from-to)243-252
Number of pages10
JournalTeratogenesis Carcinogenesis And Mutagenesis
Volume16
Issue number5
DOIs
Publication statusPublished - 1 Dec 1996

Keywords

  • Acrocentric chromosome
  • C-banding
  • Colchicine
  • CREST staining
  • Down syndrome
  • Micronuclei
  • N-banding

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