Potent bactericidal action of a flavonoid fraction isolated from the stem bark of Butea frondosa

Leonard Amaral, Mishra, Uma Shankar, Chakraborty, Prabir R., A Dasgupta , Sujata G. Dastidar, M. Martins

Research output: Contribution to journalArticlepeer-review

Abstract

The flavonoid fraction isolated from the ethyl acetate fraction (BF-1) of Butea frondosa (L.) stem bark exhibited distinct antimicrobial activity when tested against 129 bacterial strains belonging to 9 different genera of both gram-positive and gram-negative types. Minimum inhibitor), concentration (MIC) of the fraction BF-1 was determined following NCCLS guidelines using the agar dilution method. Twenty-four out of 36 strains of Staphylococcus aureus were inhibited by 50-200 mg/l of the fraction. This fraction also inhibited strains of Bacillus spp., Shigella spp., Salmonella spp. and even a few Pseudomonas at concentrations between 50-200 mg/l. Other bacteria including Escherichia coli, Vibrio cholerae and V. parahaemolyticus were moderately sensitive to BF-1. In the in vivo studies, this fraction. offered significant protection to Swiss albino mice at a concentration of 80 mu g/mouse (p<0.001) when they were challenged with 50 median lethal dose of Salmonella enteritidis NCTC 74. A fraction named BF-1 that was isolated from an ethyl acetate fraction of Butea frondosa provided protection against an infection from a Salmonella enteritidis NCTC strain.
Original languageEnglish
Pages (from-to)29-32
Number of pages4
JournalIn Vivo
VolumeVol. 23
Issue numbern.º 1
Publication statusPublished - 1 Jan 2009

Keywords

  • Butea frondosa
  • Flavonoid
  • Broad-spectrum bactericide
  • Antimicrobial activity

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