Potassium bromate oxidative DNA damage in repair proficient and deficient CHO cell lines

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Abstract

Potassium bromate (KBrO3) is a strong oxidizing agent able to induce DNA base oxidation and single strand breaks (SSBs). In the present study DNA damaging and oxidative effects of KBrO3 were evaluated in the XRCC1 deficient Chinese hamster ovary (CHO) cell line EM9 and its parental repair proficient AA8 cell line using Comet assay and 8-OHdG quantified by HPLC-ECD. Comet assay results show significant dose-response effect and an increased susceptibility of EM9 as compared to AA8 (p<0.001) after one hour of exposure. 8OHdG dose-responses are obtained for both cell lines but the EM9 increased susceptibility for KBrO3 is only significant (p<0.001) when exposure time is increased to 3 hours at the higher concentration tested.
Original languageEnglish
Title of host publicationPROCEEDINGS OF THE EUROPEAN MEETING OF THE SOCIETY FOR FREE RADICAL RESEARCH INTERNATIONAL
Pages93-97
Publication statusPublished - 1 Jan 2007
EventEuropean Meeting of the Society-for-Free-Radical-Research-International (SFRR Europe) -
Duration: 1 Jan 2007 → …

Conference

ConferenceEuropean Meeting of the Society-for-Free-Radical-Research-International (SFRR Europe)
Period1/01/07 → …

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