Potassium bromate (KBrO3) is a strong oxidizing agent able to induce DNA base oxidation and single strand breaks (SSBs). In the present study DNA damaging and oxidative effects of KBrO3 were evaluated in the XRCC1 deficient Chinese hamster ovary (CHO) cell line EM9 and its parental repair proficient AA8 cell line using Comet assay and 8-OHdG quantified by HPLC-ECD. Comet assay results show significant dose-response effect and an increased susceptibility of EM9 as compared to AA8 (p<0.001) after one hour of exposure. 8OHdG dose-responses are obtained for both cell lines but the EM9 increased susceptibility for KBrO3 is only significant (p<0.001) when exposure time is increased to 3 hours at the higher concentration tested.
|Title of host publication||PROCEEDINGS OF THE EUROPEAN MEETING OF THE SOCIETY FOR FREE RADICAL RESEARCH INTERNATIONAL|
|Publication status||Published - 1 Jan 2007|
|Event||European Meeting of the Society-for-Free-Radical-Research-International (SFRR Europe) - Vilamoura, Portugal|
Duration: 10 Oct 2007 → 13 Oct 2007
|Conference||European Meeting of the Society-for-Free-Radical-Research-International (SFRR Europe)|
|Period||10/10/07 → 13/10/07|