Abstract
Original language | English |
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Pages (from-to) | 70-78 |
Journal | Journal of Nutritional Biochemistry |
Volume | 27 |
Issue number | NA |
DOIs | |
Publication status | Published - 1 Jan 2016 |
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Postprandial insulin action relies on meal composition and hepatic parasympathetics: dependency on glucose and amino acids Meal, parasympathetics & insulin action. / Gaspar, Joana Margarida Navalho; Macedo, Maria Paula Borges de Lemos; Afonso, Ricardo Alexandre da Silva Santos.
In: Journal of Nutritional Biochemistry, Vol. 27, No. NA, 01.01.2016, p. 70-78.Research output: Contribution to journal › Article
TY - JOUR
T1 - Postprandial insulin action relies on meal composition and hepatic parasympathetics: dependency on glucose and amino acids Meal, parasympathetics & insulin action
AU - Gaspar, Joana Margarida Navalho
AU - Macedo, Maria Paula Borges de Lemos
AU - Afonso, Ricardo Alexandre da Silva Santos
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Insulin sensitivity (IS) increases following a meal. Meal composition affects postprandial glucose disposal but still remains unclear which nutrients and mechanisms are involved. We hypothesized that gut-absorbed glucose and amino acids stimulate hepatic parasympathetic nerves, potentiating insulin action. Male Sprague-Dawley rats were 24 h fasted and anesthetized. Two series of experiments were performed. (A) IS was assessed before and after liquid test meal administration (10 ml.kg(-1), intraenteric): glucose + amino acids + lipids (GAL, n=6); glucose (n=5); amino acids (n=5); lipids (n=3); glucose + amino acids (GA, n=9); amino acids + lipids (n=3); and glucose + lipids (n=4). (B) Separately, fasted animals were submitted to hepatic parasympathetic denervation (DEN); IS was assessed before and after GAL (n=4) or GA administration (n=4). (A) Both GAL and GA induced significant insulin sensitization. GAL increased IS from 97.9+/-6.2 mg glucose/kg bw (fasting) to 225.4+/-183 mg glucose/kg bw (P<0.001; 143.6+/-26.0\% potentiation of IS); GA increased IS from 109.0+/-6.6 to 240.4+/-18.0 mg glucose/kg bw (P<0.001; 123.1+/-13.4\% potentiation). None of the other meals potentiated IS. (B) GAL and GA did not induce a significant insulin sensitization in DEN animal. To achieve maximal insulin sensitization following a meal, it is required that gut-absorbed glucose and amino acids trigger a vagal reflex that involves hepatic parasympathetic nerves. (C) 2015 Elsevier Inc. All rights reserved.
AB - Insulin sensitivity (IS) increases following a meal. Meal composition affects postprandial glucose disposal but still remains unclear which nutrients and mechanisms are involved. We hypothesized that gut-absorbed glucose and amino acids stimulate hepatic parasympathetic nerves, potentiating insulin action. Male Sprague-Dawley rats were 24 h fasted and anesthetized. Two series of experiments were performed. (A) IS was assessed before and after liquid test meal administration (10 ml.kg(-1), intraenteric): glucose + amino acids + lipids (GAL, n=6); glucose (n=5); amino acids (n=5); lipids (n=3); glucose + amino acids (GA, n=9); amino acids + lipids (n=3); and glucose + lipids (n=4). (B) Separately, fasted animals were submitted to hepatic parasympathetic denervation (DEN); IS was assessed before and after GAL (n=4) or GA administration (n=4). (A) Both GAL and GA induced significant insulin sensitization. GAL increased IS from 97.9+/-6.2 mg glucose/kg bw (fasting) to 225.4+/-183 mg glucose/kg bw (P<0.001; 143.6+/-26.0\% potentiation of IS); GA increased IS from 109.0+/-6.6 to 240.4+/-18.0 mg glucose/kg bw (P<0.001; 123.1+/-13.4\% potentiation). None of the other meals potentiated IS. (B) GAL and GA did not induce a significant insulin sensitization in DEN animal. To achieve maximal insulin sensitization following a meal, it is required that gut-absorbed glucose and amino acids trigger a vagal reflex that involves hepatic parasympathetic nerves. (C) 2015 Elsevier Inc. All rights reserved.
KW - Glucose
KW - HEART-RATE-VARIABILITY
KW - NITRIC-OXIDE
KW - PANCREATIC-SECRETION
KW - Parasympathetic nerves
KW - Protein intake
KW - Insulin sensitivity
KW - Insulin action
KW - EUGLYCEMIC CLAMP
KW - IMPROVES GLUCOSE
KW - DIABETES-MELLITUS
KW - TOLERANCE-TEST
KW - OBESE ZUCKER RAT
KW - BRAIN-GUT AXIS
KW - Nutritional modulation of insulin sensitivity
KW - Meal composition
KW - VAGUS NERVE
U2 - 10.1016/j.jnutbio.2015.08.023
DO - 10.1016/j.jnutbio.2015.08.023
M3 - Article
VL - 27
SP - 70
EP - 78
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
SN - 0955-2863
IS - NA
ER -