The reversed horizontal cyclic loading test of a flat slab specimen with shear stud reinforcement is described. The purpose is to investigate the post-earthquake strength and deformation capacity of the flat slab–column assembly. Shear reinforcement consisted of 5 perimeters of 8 mm bars welded into rectangular steel bars that served for anchorage and to facilitate installation. The test was conducted in two phases to simulate a scenario in which an already damaged flat slab–column assembly is subjected to a strong earthquake. In the first phase, the slab was loaded with a vertical load corresponding to an approximately 55% gravity shear ratio. Subsequently, the horizontal loading protocol was followed up to 3% drift ratio. At the end of this stage, the specimen was considerably cracked and the unbalanced moment–drift relationship had reached an almost horizontal plateau. The slab was unloaded and loaded again for the second phase, in which the horizontal loading protocol was restarted and followed up to failure. The reloading phase (second phase) was characterized by a reduced loading stiffness and lower horizontal forces compared to the first phase. However, the horizontal force was gradually increased with horizontal drifts until it reached the value obtained in the first phase, resulting in an envelope horizontal force–displacement relationship similar to that of previous similar specimens tested up to failure in a single phase. The specimen continued to sustain the gravity load and the horizontal force up to 5.5% drift.